Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM7092693	M	M	NA	Agree	1.38	12.29	0.00	17.30	14.17	20.26	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.454617	0.476464	0.067543	0.001376	84.71	2.43	11.26	2529	LP2 (2026-09-30)	PASS	NonPolyA_perM 2529 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7092694	M	M	NA	Agree	1.07	11.52	0.00	16.87	13.83	19.19	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.450734	0.479025	0.068782	0.001459	85.26	2.44	10.69	2423	LP2 (2026-09-30)	PASS	NonPolyA_perM 2423 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7092695	M	M	NA	Agree	1.51	16.11	0.00	21.18	17.39	23.77	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.448473	0.483252	0.063531	0.004745	83.97	2.52	12.17	2104	LP2 (2026-09-30)	PASS	NonPolyA_perM 2104 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7092696	M	M	NA	Agree	1.03	10.74	0.00	18.06	13.32	17.69	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.463039	0.466189	0.068925	0.001848	83.48	2.60	12.11	2885	LP2 (2026-09-30)	PASS	NonPolyA_perM 2885 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7092697	M	M	NA	Agree	1.83	14.74	0.00	14.44	13.78	19.27	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.452868	0.482282	0.063559	0.001291	84.05	2.72	11.48	2838	LP2 (2026-09-30)	PASS	NonPolyA_perM 2838 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM7092698	M	M	NA	Agree	1.76	16.29	0.00	17.58	13.79	20.81	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.446014	0.493303	0.059812	0.000872	84.20	2.18	12.08	2326	LP2 (2026-09-30)	PASS	NonPolyA_perM 2326 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not