Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM7058762	M	M	NA	Agree	0.00	2.42	0.00	8.26	3.75	33.88	NONE	POLYA	0.941961	0.032811	0.022066	0.003161	94.00	2.00	3.84	37	LP2 (2026-09-30)	PASS	NonPolyA_perM 37 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7058763	M	M	NA	Agree	0.00	1.55	0.00	9.47	3.69	39.18	NONE	POLYA	0.949038	0.026208	0.021123	0.003631	93.92	2.04	3.90	38	LP2 (2026-09-30)	PASS	NonPolyA_perM 38 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7058764	M	M	NA	Agree	0.00	1.98	0.00	9.01	3.64	38.75	NONE	POLYA	0.943947	0.030922	0.021864	0.003267	94.09	1.97	3.79	37	LP2 (2026-09-30)	PASS	NonPolyA_perM 37 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7058765	M	M	NA	Agree	0.00	1.66	0.00	7.31	3.05	35.63	NONE	POLYA	0.9436	0.028108	0.021137	0.007155	93.80	2.44	3.58	33	LP2 (2026-09-30)	PASS	NonPolyA_perM 33 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7058766	M	M	NA	Agree	0.00	2.37	0.00	9.04	4.70	39.52	NONE	POLYA	0.945069	0.029962	0.022589	0.002379	93.69	1.92	4.24	36	LP2 (2026-09-30)	PASS	NonPolyA_perM 36 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7058767	M	M	NA	Agree	0.00	2.03	0.00	10.54	5.42	39.44	NONE	POLYA	0.940446	0.034049	0.022455	0.00305	93.43	1.93	4.49	45	LP2 (2026-09-30)	PASS	NonPolyA_perM 45 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7058773	M	M	NA	Agree	0.00	1.79	0.00	9.39	3.31	41.10	NONE	POLYA	0.94588	0.029121	0.02135	0.003649	94.35	1.96	3.53	34	LP2 (2026-09-30)	PASS	NonPolyA_perM 34 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7058774	M	M	NA	Agree	0.00	1.73	0.00	7.64	4.28	39.82	NONE	POLYA	0.94541	0.029367	0.021607	0.003617	94.14	1.98	3.71	41	LP2 (2026-09-30)	PASS	NonPolyA_perM 41 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7058775	M	M	NA	Agree	0.00	1.72	0.00	9.42	4.50	41.54	NONE	POLYA	0.942679	0.032015	0.022663	0.002643	93.90	1.89	4.04	36	LP2 (2026-09-30)	PASS	NonPolyA_perM 36 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7058776	M	M	NA	Agree	0.00	1.80	0.00	10.20	4.33	42.90	NONE	POLYA	0.944449	0.029964	0.022458	0.003129	94.38	1.99	3.45	38	LP2 (2026-09-30)	PASS	NonPolyA_perM 38 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7058777	M	M	NA	Agree	0.00	1.68	0.00	7.77	3.96	39.72	NONE	POLYA	0.948561	0.027611	0.020802	0.003026	94.26	1.92	3.66	30	LP2 (2026-09-30)	PASS	NonPolyA_perM 30 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7058778	M	M	NA	Agree	0.00	1.58	0.00	7.51	3.91	36.12	NONE	POLYA	0.951161	0.025047	0.020955	0.002837	93.88	1.90	4.06	28	LP2 (2026-09-30)	PASS	NonPolyA_perM 28 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does