Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM7047582	M	F	NA	Conflict	27.69	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.907612	0.039423	0.023718	0.029247	87.71	4.52	7.57	52	LP2 (2026-09-30)	PASS	NonPolyA_perM 52 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7047583	M	M	NA	Agree	0.05	0.60	0.00	4.63	3.55	8.91	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.927818	0.028299	0.018303	0.025579	87.55	4.23	8.03	32	LP2 (2026-09-30)	PASS	NonPolyA_perM 32 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7047584	M	M	NA	Agree	0.11	1.87	0.00	7.51	5.76	17.72	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.907043	0.034327	0.023785	0.034844	85.94	5.16	8.72	43	LP2 (2026-09-30)	PASS	NonPolyA_perM 43 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7047585	M	M	NA	Agree	0.06	0.98	0.00	4.41	4.34	15.89	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.939278	0.028289	0.02118	0.011253	88.81	3.04	7.95	73	LP2 (2026-09-30)	PASS	NonPolyA_perM 73 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7047586	M	M	NA	Agree	0.04	1.56	0.00	15.81	5.55	35.80	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.920297	0.038128	0.027082	0.014493	87.44	3.94	8.33	100	LP2 (2026-09-30)	PASS	NonPolyA_perM 100 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7047587	M	M	NA	Agree	0.03	1.07	0.00	7.07	4.10	16.38	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.926172	0.033025	0.021236	0.019567	88.13	3.84	7.81	97	LP2 (2026-09-30)	PASS	NonPolyA_perM 97 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does