Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM7040104	M	M	NA	Agree	0.00	2.83	0.00	12.40	3.25	32.28	NONE	POLYA	0.95798	0.016605	0.017681	0.007733	90.06	2.20	7.63	35	LP2 (2026-09-30)	PASS	NonPolyA_perM 35 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7040105	M	M	NA	Agree	0.00	0.85	0.00	4.15	1.38	15.06	NONE	POLYA	0.953356	0.022861	0.022193	0.00159	93.11	2.11	4.68	63	LP2 (2026-09-30)	PASS	NonPolyA_perM 63 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7040106	M	M	NA	Agree	0.00	2.96	0.00	13.22	2.37	36.84	NONE	POLYA	0.961339	0.013377	0.015634	0.009651	90.65	2.29	6.97	20	LP2 (2026-09-30)	PASS	NonPolyA_perM 20 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7040107	M	M	NA	Agree	0.00	3.27	0.00	11.95	4.78	26.03	NONE	POLYA	0.944249	0.025982	0.027473	0.002296	91.82	2.27	5.68	46	LP2 (2026-09-30)	PASS	NonPolyA_perM 46 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7040108	M	M	NA	Agree	0.00	3.28	0.00	13.42	3.95	37.04	NONE	POLYA	0.960047	0.01593	0.016865	0.007158	90.30	1.89	7.72	23	LP2 (2026-09-30)	PASS	NonPolyA_perM 23 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7040109	M	M	NA	Agree	0.00	2.17	0.00	8.54	3.16	22.72	NONE	POLYA	0.952754	0.015619	0.018067	0.013559	90.53	3.19	6.15	25	LP2 (2026-09-30)	PASS	NonPolyA_perM 25 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7040110	M	M	NA	Agree	0.00	2.69	0.00	14.53	4.33	32.18	NONE	POLYA	0.959446	0.016129	0.019239	0.005187	91.96	1.84	6.09	27	LP2 (2026-09-30)	PASS	NonPolyA_perM 27 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7040111	M	M	NA	Agree	0.00	6.62	0.00	22.10	5.81	54.14	NONE	POLYA	0.952171	0.016921	0.020805	0.010102	86.59	2.03	11.29	27	LP2 (2026-09-30)	PASS	NonPolyA_perM 27 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7040112	M	M	NA	Agree	0.00	2.15	0.00	8.96	2.81	34.61	NONE	POLYA	0.952969	0.015822	0.017904	0.013305	89.72	2.67	7.48	34	LP2 (2026-09-30)	PASS	NonPolyA_perM 34 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7040113	M	M	NA	Agree	0.00	1.87	0.00	8.57	2.40	32.14	NONE	POLYA	0.956527	0.014005	0.017682	0.011786	91.92	2.48	5.48	37	LP2 (2026-09-30)	PASS	NonPolyA_perM 37 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7040114	M	M	NA	Agree	0.00	2.55	0.00	8.40	3.26	30.03	NONE	POLYA	0.962189	0.015061	0.018563	0.004187	89.99	1.86	8.06	30	LP2 (2026-09-30)	PASS	NonPolyA_perM 30 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does