Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM7036491	M	M	NA	Agree	0.00	5.63	0.00	25.36	13.22	60.03	NONE	POLYA	0.838724	0.087002	0.035035	0.039239	92.04	5.53	2.26	84	LP2 (2026-09-30)	PASS	NonPolyA_perM 84 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7036492	M	M	NA	Agree	0.04	6.43	0.27	23.88	12.71	59.16	NONE	POLYA	0.87031	0.084125	0.036009	0.009556	94.92	2.74	2.18	73	LP2 (2026-09-30)	PASS	NonPolyA_perM 73 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7036493	M	M	NA	Agree	0.01	5.97	0.00	23.79	10.43	60.38	NONE	POLYA	0.88384	0.075439	0.034238	0.006483	95.30	2.44	2.11	71	LP2 (2026-09-30)	PASS	NonPolyA_perM 71 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7036494	M	M	NA	Agree	0.01	6.96	0.00	26.38	13.81	61.91	NONE	POLYA	0.885391	0.075108	0.034849	0.004652	95.37	2.29	2.18	63	LP2 (2026-09-30)	PASS	NonPolyA_perM 63 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7036495	M	M	NA	Agree	0.01	6.60	0.00	27.51	11.90	52.52	NONE	POLYA	0.87862	0.079928	0.035078	0.006373	95.49	2.48	1.86	75	LP2 (2026-09-30)	PASS	NonPolyA_perM 75 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7036496	M	M	NA	Agree	0.01	5.25	0.00	24.98	12.72	62.81	NONE	POLYA	0.878349	0.081516	0.035241	0.004893	95.59	2.18	2.06	76	LP2 (2026-09-30)	PASS	NonPolyA_perM 76 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7036497	M	M	NA	Agree	0.03	7.26	0.00	25.96	11.72	63.33	NONE	POLYA	0.883351	0.076343	0.035355	0.00495	95.46	2.17	2.21	59	LP2 (2026-09-30)	PASS	NonPolyA_perM 59 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7036498	M	M	NA	Agree	0.01	7.17	0.00	26.62	12.04	61.47	NONE	POLYA	0.847208	0.076817	0.032271	0.043704	92.01	5.63	2.21	66	LP2 (2026-09-30)	PASS	NonPolyA_perM 66 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7036499	M	M	NA	Agree	0.02	7.12	0.00	25.40	11.99	58.12	NONE	POLYA	0.849709	0.078471	0.033529	0.038291	92.50	5.18	2.16	80	LP2 (2026-09-30)	PASS	NonPolyA_perM 80 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7036500	M	M	NA	Agree	0.02	6.92	0.00	26.48	15.20	77.21	NONE	POLYA	0.863262	0.094105	0.037023	0.005611	95.38	2.29	2.17	91	LP2 (2026-09-30)	PASS	NonPolyA_perM 91 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7036501	M	M	NA	Agree	0.05	7.71	0.00	30.24	14.28	67.14	NONE	POLYA	0.860869	0.091309	0.03818	0.009642	94.98	2.59	2.25	84	LP2 (2026-09-30)	PASS	NonPolyA_perM 84 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7036502	M	M	NA	Agree	0.05	6.84	0.00	30.85	14.18	66.92	NONE	POLYA	0.859867	0.095602	0.03849	0.00604	95.03	2.16	2.63	86	LP2 (2026-09-30)	PASS	NonPolyA_perM 86 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7036503	M	M	NA	Agree	0.01	5.98	0.00	24.08	11.41	60.40	NONE	POLYA	0.865489	0.092655	0.038057	0.003799	95.53	2.01	2.28	86	LP2 (2026-09-30)	PASS	NonPolyA_perM 86 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7036504	M	M	NA	Agree	0.06	4.82	0.00	25.14	14.48	57.46	NONE	POLYA	0.870239	0.089383	0.037297	0.003081	95.33	2.06	2.41	82	LP2 (2026-09-30)	PASS	NonPolyA_perM 82 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7036505	M	M	NA	Agree	0.04	6.43	0.00	25.62	14.01	68.00	NONE	POLYA	0.870831	0.088211	0.036159	0.0048	94.97	2.22	2.60	79	LP2 (2026-09-30)	PASS	NonPolyA_perM 79 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7036506	M	M	NA	Agree	0.02	8.10	0.00	32.26	15.70	75.40	NONE	POLYA	0.811101	0.131125	0.045192	0.012582	94.82	2.85	2.08	125	LP2 (2026-09-30)	PASS	NonPolyA_perM 125 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7036507	M	M	NA	Agree	0.00	5.10	0.00	25.69	11.03	64.60	NONE	POLYA	0.816977	0.09553	0.045799	0.041694	90.89	6.27	2.39	95	LP2 (2026-09-30)	PASS	NonPolyA_perM 95 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7036508	M	M	NA	Agree	0.04	7.09	0.00	29.19	12.75	71.58	NONE	POLYA	0.822078	0.114975	0.045054	0.017894	94.11	3.49	2.10	88	LP2 (2026-09-30)	PASS	NonPolyA_perM 88 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7036509	M	M	NA	Agree	0.03	7.60	0.00	31.17	12.57	73.82	NONE	POLYA	0.796055	0.122818	0.046579	0.034548	92.20	5.17	2.35	125	LP2 (2026-09-30)	PASS	NonPolyA_perM 125 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7036510	M	M	NA	Agree	0.00	8.12	0.00	32.64	15.35	80.52	NONE	POLYA	0.821077	0.10566	0.041102	0.032161	93.08	4.43	2.31	117	LP2 (2026-09-30)	PASS	NonPolyA_perM 117 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7036511	M	M	NA	Agree	0.00	5.00	0.00	29.11	11.20	84.03	NONE	POLYA	0.848837	0.098723	0.046236	0.006204	94.52	2.65	2.30	97	LP2 (2026-09-30)	PASS	NonPolyA_perM 97 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7036512	M	M	NA	Agree	0.00	5.28	0.00	20.87	12.09	81.44	NONE	POLYA	0.880226	0.07219	0.044646	0.002939	95.28	2.17	2.22	68	LP2 (2026-09-30)	PASS	NonPolyA_perM 68 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7036513	M	M	NA	Agree	0.00	6.11	0.00	25.16	13.49	82.16	NONE	POLYA	0.858719	0.076993	0.040285	0.024004	93.68	3.82	2.23	95	LP2 (2026-09-30)	PASS	NonPolyA_perM 95 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7036514	M	M	NA	Agree	0.01	7.73	0.00	31.34	13.63	86.52	NONE	POLYA	0.848309	0.103979	0.043503	0.00421	95.13	2.23	2.26	103	LP2 (2026-09-30)	PASS	NonPolyA_perM 103 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7036515	M	M	NA	Agree	0.00	15.96	0.00	29.23	21.03	61.45	NONE	POLYA	0.661027	0.270754	0.049896	0.018323	88.46	4.24	6.94	193	LP2 (2026-09-30)	PASS	NonPolyA_perM 193 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7036516	M	M	NA	Agree	0.03	7.57	0.00	31.79	15.62	81.84	NONE	POLYA	0.833208	0.118442	0.043138	0.005213	95.18	2.12	2.39	95	LP2 (2026-09-30)	PASS	NonPolyA_perM 95 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7036517	M	M	NA	Agree	0.11	6.95	0.00	28.63	15.39	70.04	NONE	POLYA	0.833079	0.114374	0.047245	0.005303	94.86	2.34	2.43	107	LP2 (2026-09-30)	PASS	NonPolyA_perM 107 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7036518	M	M	NA	Agree	0.00	5.29	0.00	24.25	12.92	87.40	NONE	POLYA	0.873586	0.07668	0.045353	0.004382	94.72	2.38	2.48	70	LP2 (2026-09-30)	PASS	NonPolyA_perM 70 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7036519	M	M	NA	Agree	0.00	5.76	0.00	23.32	12.10	82.54	NONE	POLYA	0.875376	0.07601	0.044082	0.004532	94.45	2.56	2.56	87	LP2 (2026-09-30)	PASS	NonPolyA_perM 87 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM7036520	M	M	NA	Agree	0.02	5.91	0.00	24.75	12.20	75.27	NONE	POLYA	0.863985	0.078999	0.045583	0.011434	93.82	3.20	2.53	81	LP2 (2026-09-30)	PASS	NonPolyA_perM 81 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does