Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM6924955	M	M	NA	Agree	0.02	8.46	0.00	44.01	20.63	74.82	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.872722	0.080109	0.045851	0.001317	90.42	1.36	8.04	71	LP2 (2026-09-30)	PASS	NonPolyA_perM 71 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6924956	M	M	NA	Agree	0.05	6.43	0.00	43.45	21.28	64.11	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.863561	0.089208	0.044257	0.002974	88.50	1.44	9.89	75	LP2 (2026-09-30)	PASS	NonPolyA_perM 75 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6924957	M	M	NA	Agree	0.00	8.55	0.00	50.11	19.35	68.57	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.869397	0.087326	0.042939	0.000338	89.44	1.21	9.18	81	LP2 (2026-09-30)	PASS	NonPolyA_perM 81 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does