Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-20) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM6910738	not_specified	M	NA	Conflict	0.01	8.57	0.00	50.42	20.70	80.92	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.848492	0.08648	0.03923	0.025798	92.28	5.30	2.16	129	LP2 (2026-09-30)	PASS	NonPolyA_perM 129 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6910739	not_specified	M	NA	Conflict	0.01	6.07	0.00	43.04	19.45	73.21	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.860232	0.082082	0.040502	0.017184	92.85	4.74	2.08	97	LP2 (2026-09-30)	PASS	NonPolyA_perM 97 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6910740	not_specified	M	NA	Conflict	0.05	7.07	0.00	40.46	18.67	80.64	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.844218	0.080569	0.040769	0.034444	90.66	6.24	2.81	108	LP2 (2026-09-30)	PASS	NonPolyA_perM 108 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6910741	not_specified	M	NA	Conflict	0.00	1.85	0.00	16.07	7.71	46.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.915854	0.012662	0.030928	0.040556	89.13	7.16	3.44	15	LP2 (2026-09-30)	PASS	NonPolyA_perM 15 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6910742	not_specified	M	NA	Conflict	0.01	8.00	0.00	47.36	21.68	75.42	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.861117	0.080082	0.03958	0.019221	93.28	4.57	1.90	104	LP2 (2026-09-30)	PASS	NonPolyA_perM 104 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6910743	not_specified	M	NA	Conflict	0.04	7.61	0.00	52.57	23.49	86.58	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.848259	0.086532	0.042052	0.023157	92.65	4.96	2.10	104	LP2 (2026-09-30)	PASS	NonPolyA_perM 104 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6910744	not_specified	M	NA	Conflict	0.06	8.81	0.00	53.14	22.96	80.77	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.848565	0.086521	0.039874	0.02504	93.02	4.94	1.78	130	LP2 (2026-09-30)	PASS	NonPolyA_perM 130 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6910745	not_specified	M	NA	Conflict	0.00	3.89	0.00	20.16	9.05	49.59	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.894144	0.015416	0.031715	0.058724	87.27	8.60	3.84	19	LP2 (2026-09-30)	PASS	NonPolyA_perM 19 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6910746	not_specified	M	NA	Conflict	0.00	2.80	0.00	23.36	6.79	33.29	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.907284	0.015081	0.027248	0.050388	88.39	7.56	3.76	12	LP2 (2026-09-30)	PASS	NonPolyA_perM 12 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6910747	not_specified	M	NA	Conflict	0.01	3.30	0.00	49.52	8.95	52.96	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.9069	0.044017	0.024744	0.024338	93.17	4.83	1.67	43	LP2 (2026-09-30)	PASS	NonPolyA_perM 43 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6910748	not_specified	M	NA	Conflict	0.02	5.35	0.00	61.16	11.51	52.51	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.904777	0.052213	0.028952	0.014058	93.81	3.82	2.06	49	LP2 (2026-09-30)	PASS	NonPolyA_perM 49 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6910749	not_specified	M	NA	Conflict	0.01	4.12	0.00	47.24	5.68	58.32	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.936021	0.028539	0.022636	0.012805	94.51	3.68	1.55	23	LP2 (2026-09-30)	PASS	NonPolyA_perM 23 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6910750	not_specified	M	NA	Conflict	0.00	4.51	0.00	28.67	11.69	52.09	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.9344	0.013446	0.032018	0.020135	91.08	4.71	3.91	12	LP2 (2026-09-30)	PASS	NonPolyA_perM 12 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6910751	not_specified	M	NA	Conflict	0.04	8.76	0.00	54.61	23.57	81.70	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.861221	0.086711	0.040218	0.01185	94.29	3.62	1.82	121	LP2 (2026-09-30)	PASS	NonPolyA_perM 121 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6910752	not_specified	M	NA	Conflict	0.09	6.65	0.00	49.59	19.60	71.67	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.859791	0.082024	0.039741	0.018443	92.82	4.82	2.08	108	LP2 (2026-09-30)	PASS	NonPolyA_perM 108 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6910753	not_specified	M	NA	Conflict	0.06	6.88	0.00	51.58	19.06	71.36	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.868046	0.076814	0.038737	0.016403	93.20	3.96	2.54	106	LP2 (2026-09-30)	PASS	NonPolyA_perM 106 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does