Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM6797507	F	F	NA	Agree	65.62	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.936833	0.026186	0.033614	0.003367	88.14	2.01	9.68	28	LP2 (2026-09-30)	PASS	NonPolyA_perM 28 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6797508	F	F	NA	Agree	58.52	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.937468	0.025548	0.031737	0.005248	87.64	2.29	9.86	40	LP2 (2026-09-30)	PASS	NonPolyA_perM 40 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6797509	F	F	NA	Agree	47.65	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.927048	0.024362	0.030621	0.017968	88.07	3.31	8.46	48	LP2 (2026-09-30)	PASS	NonPolyA_perM 48 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6797510	F	F	NA	Agree	42.32	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.942874	0.022085	0.032257	0.002783	88.22	2.05	9.57	45	LP2 (2026-09-30)	PASS	NonPolyA_perM 45 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6797511	F	F	NA	Agree	41.34	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.943153	0.02117	0.030563	0.005114	88.13	2.31	9.39	34	LP2 (2026-09-30)	PASS	NonPolyA_perM 34 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6797512	F	F	NA	Agree	56.16	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.917909	0.02551	0.031748	0.024832	87.21	4.08	8.56	46	LP2 (2026-09-30)	PASS	NonPolyA_perM 46 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does