Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-22) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM6684669	M	M	NA	Agree	0.03	7.46	0.00	25.30	10.65	54.57	NONE	POLYA	0.901789	0.054603	0.036268	0.00734	93.01	3.48	2.91	110	LP2 (2026-09-30)	PASS	NonPolyA_perM 110 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6684670	M	M	NA	Agree	0.06	6.89	0.00	29.87	11.41	53.14	NONE	POLYA	0.901587	0.058276	0.034164	0.005973	93.97	3.14	2.44	90	LP2 (2026-09-30)	PASS	NonPolyA_perM 90 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6684671	M	M	NA	Agree	0.07	7.44	0.00	26.89	13.15	54.05	NONE	POLYA	0.901941	0.057161	0.035464	0.005433	92.87	2.92	3.77	99	LP2 (2026-09-30)	PASS	NonPolyA_perM 99 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6684672	M	M	NA	Agree	0.08	5.57	0.00	23.87	10.21	42.58	NONE	POLYA	0.913304	0.049688	0.029905	0.007103	93.31	3.51	2.45	105	LP2 (2026-09-30)	PASS	NonPolyA_perM 105 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6684673	M	M	NA	Agree	0.07	6.93	0.00	24.77	8.97	51.23	NONE	POLYA	0.912718	0.047825	0.032677	0.00678	92.49	3.11	3.92	66	LP2 (2026-09-30)	PASS	NonPolyA_perM 66 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6684674	M	M	NA	Agree	0.10	8.72	0.00	32.76	13.49	55.31	NONE	POLYA	0.899128	0.060786	0.035681	0.004405	92.66	2.84	3.90	76	LP2 (2026-09-30)	PASS	NonPolyA_perM 76 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6684675	M	M	NA	Agree	0.04	7.70	0.00	25.26	11.27	55.41	NONE	POLYA	0.905398	0.049794	0.039198	0.00561	91.28	3.40	4.89	118	LP2 (2026-09-30)	PASS	NonPolyA_perM 118 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6684676	M	M	NA	Agree	0.09	9.00	0.00	27.50	12.16	48.61	NONE	POLYA	0.907833	0.057571	0.030932	0.003664	90.86	3.80	4.60	100	LP2 (2026-09-30)	PASS	NonPolyA_perM 100 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6684677	M	M	NA	Agree	0.11	9.69	0.00	31.42	14.47	60.20	NONE	POLYA	0.900036	0.061736	0.033062	0.005166	92.48	2.85	4.16	93	LP2 (2026-09-30)	PASS	NonPolyA_perM 93 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does