Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM6523104	M	M	NA	Agree	0.35	9.76	0.00	51.52	22.70	50.41	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.866856	0.099296	0.03201	0.001838	94.13	3.62	1.94	168	LP2 (2026-09-30)	PASS	NonPolyA_perM 168 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6523105	M	M	NA	Agree	0.46	11.24	0.00	53.11	21.15	51.73	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.874942	0.091198	0.031476	0.002383	94.03	3.61	2.02	138	LP2 (2026-09-30)	PASS	NonPolyA_perM 138 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6523106	M	M	NA	Agree	0.33	9.87	0.00	47.66	19.81	50.40	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.862388	0.102835	0.032185	0.002591	93.75	3.85	2.08	156	LP2 (2026-09-30)	PASS	NonPolyA_perM 156 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6523107	M	M	NA	Agree	0.41	7.74	0.00	45.69	19.61	47.44	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.871286	0.094984	0.032156	0.001574	94.39	3.51	1.80	148	LP2 (2026-09-30)	PASS	NonPolyA_perM 148 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6523108	M	M	NA	Agree	0.43	8.64	0.00	48.15	18.74	50.02	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.877437	0.088624	0.03159	0.00235	94.41	3.54	1.75	163	LP2 (2026-09-30)	PASS	NonPolyA_perM 163 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6523109	M	M	NA	Agree	0.42	11.17	0.00	60.99	22.95	58.14	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.856606	0.106951	0.034011	0.002432	94.33	3.41	1.95	164	LP2 (2026-09-30)	PASS	NonPolyA_perM 164 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6744022	M	M	NA	Agree	0.49	13.16	0.00	61.94	22.02	52.20	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.868394	0.096336	0.031633	0.003637	93.73	3.99	1.95	174	LP2 (2026-09-30)	PASS	NonPolyA_perM 174 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6744023	M	M	NA	Agree	0.25	8.37	0.00	42.50	18.67	47.04	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.868186	0.097894	0.031526	0.002394	93.37	4.19	2.08	185	LP2 (2026-09-30)	PASS	NonPolyA_perM 185 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6744024	M	M	NA	Agree	0.25	9.17	0.00	43.66	19.16	46.38	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.866704	0.099841	0.03134	0.002115	92.97	4.58	2.10	148	LP2 (2026-09-30)	PASS	NonPolyA_perM 148 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does