Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-15) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM6507594	M	M	NA	Agree	0.02	2.95	0.00	20.02	4.62	29.60	NONE	POLYA	0.937167	0.030709	0.018599	0.013525	93.68	3.05	2.94	31	LP2 (2026-09-30)	PASS	NonPolyA_perM 31 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6507595	M	M	NA	Agree	0.02	1.95	0.00	23.54	3.64	26.17	NONE	POLYA	0.916341	0.02666	0.018635	0.038364	91.35	5.54	2.85	33	LP2 (2026-09-30)	PASS	NonPolyA_perM 33 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6507596	M	M	NA	Agree	0.02	1.87	0.00	22.31	3.73	32.42	NONE	POLYA	0.928569	0.024472	0.018713	0.028246	92.36	4.59	2.75	22	LP2 (2026-09-30)	PASS	NonPolyA_perM 22 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6507597	M	M	NA	Agree	0.01	2.51	0.00	21.57	4.47	33.03	NONE	POLYA	0.934464	0.023621	0.01904	0.022875	92.73	4.27	2.67	32	LP2 (2026-09-30)	PASS	NonPolyA_perM 32 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6507598	M	M	NA	Agree	0.01	2.79	0.00	21.61	4.18	24.50	NONE	POLYA	0.941405	0.024421	0.018377	0.015797	93.43	3.55	2.69	28	LP2 (2026-09-30)	PASS	NonPolyA_perM 28 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6507599	M	M	NA	Agree	0.01	2.39	0.00	25.10	4.64	31.49	NONE	POLYA	0.943243	0.030766	0.020604	0.005387	94.30	2.53	2.83	46	LP2 (2026-09-30)	PASS	NonPolyA_perM 46 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6507600	M	M	NA	Agree	0.01	2.13	0.00	21.14	4.02	29.52	NONE	POLYA	0.94488	0.024782	0.019566	0.010772	93.77	2.87	3.05	29	LP2 (2026-09-30)	PASS	NonPolyA_perM 29 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6507601	M	M	NA	Agree	0.00	2.75	0.00	24.01	3.82	25.48	NONE	POLYA	0.953114	0.023559	0.018526	0.004801	93.42	2.51	3.68	25	LP2 (2026-09-30)	PASS	NonPolyA_perM 25 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does