Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM6437761	M	M	NA	Agree	0.00	1.01	0.00	8.88	4.39	24.66	NONE	POLYA	0.941314	0.032379	0.020663	0.005644	95.80	1.91	2.20	38	LP2 (2026-09-30)	PASS	NonPolyA_perM 38 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6437762	M	M	NA	Agree	0.00	0.25	0.00	2.13	1.04	6.58	NONE	POLYA	0.966446	0.014298	0.013001	0.006254	95.93	1.84	2.14	11	LP2 (2026-09-30)	PASS	NonPolyA_perM 11 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6437763	M	M	NA	Agree	0.00	1.63	0.00	13.43	3.61	39.36	NONE	POLYA	0.942219	0.031669	0.020412	0.005701	95.85	1.93	2.11	41	LP2 (2026-09-30)	PASS	NonPolyA_perM 41 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6437764	M	M	NA	Agree	0.00	1.32	0.00	13.81	4.84	43.24	NONE	POLYA	0.93995	0.033085	0.021399	0.005566	96.01	1.91	1.98	41	LP2 (2026-09-30)	PASS	NonPolyA_perM 41 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6437765	M	M	NA	Agree	0.02	2.33	0.00	13.17	5.45	49.22	NONE	POLYA	0.933635	0.036571	0.023361	0.006433	95.14	2.04	2.72	47	LP2 (2026-09-30)	PASS	NonPolyA_perM 47 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6437766	M	M	NA	Agree	0.00	2.55	0.00	18.42	5.55	43.56	NONE	POLYA	0.930034	0.040202	0.024087	0.005676	95.75	2.06	2.09	47	LP2 (2026-09-30)	PASS	NonPolyA_perM 47 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6437767	M	M	NA	Agree	0.00	2.52	0.00	14.65	4.64	48.33	NONE	POLYA	0.93427	0.035254	0.02349	0.006986	95.66	2.13	2.10	37	LP2 (2026-09-30)	PASS	NonPolyA_perM 37 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6437768	M	M	NA	Agree	0.00	2.44	0.00	14.26	4.87	42.88	NONE	POLYA	0.937597	0.034448	0.02298	0.004975	95.25	2.08	2.55	49	LP2 (2026-09-30)	PASS	NonPolyA_perM 49 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6437769	M	M	NA	Agree	0.00	2.69	0.00	15.03	5.14	45.48	NONE	POLYA	0.931993	0.03592	0.025433	0.006654	95.63	2.19	2.07	49	LP2 (2026-09-30)	PASS	NonPolyA_perM 49 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6437770	M	M	NA	Agree	0.00	2.49	0.00	14.80	4.17	42.69	NONE	POLYA	0.934734	0.036246	0.023708	0.005312	95.69	1.97	2.23	53	LP2 (2026-09-30)	PASS	NonPolyA_perM 53 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6437771	M	M	NA	Agree	0.00	2.56	0.00	14.16	5.16	52.26	NONE	POLYA	0.926924	0.038203	0.026956	0.007916	95.50	2.36	2.04	65	LP2 (2026-09-30)	PASS	NonPolyA_perM 65 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6437772	M	M	NA	Agree	0.03	1.90	0.00	18.65	5.61	45.26	NONE	POLYA	0.934732	0.036635	0.023322	0.005311	95.70	2.04	2.16	51	LP2 (2026-09-30)	PASS	NonPolyA_perM 51 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6437773	M	M	NA	Agree	0.00	0.72	0.00	7.20	1.66	18.02	NONE	POLYA	0.971921	0.011748	0.0115	0.004831	96.23	1.74	1.96	4	LP2 (2026-09-30)	PASS	NonPolyA_perM 4 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6437774	M	M	NA	Agree	0.00	2.96	0.00	29.43	4.43	44.20	NONE	POLYA	0.950573	0.029609	0.01759	0.002228	96.19	1.60	2.10	32	LP2 (2026-09-30)	PASS	NonPolyA_perM 32 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6437775	M	M	NA	Agree	0.00	0.59	0.00	6.55	1.46	17.19	NONE	POLYA	0.972922	0.011714	0.012246	0.003118	96.49	1.56	1.84	9	LP2 (2026-09-30)	PASS	NonPolyA_perM 9 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6437776	M	M	NA	Agree	0.00	2.63	0.00	26.33	3.55	40.17	NONE	POLYA	0.952721	0.026335	0.01796	0.002984	96.23	1.67	2.01	24	LP2 (2026-09-30)	PASS	NonPolyA_perM 24 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does