Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-22) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM6432648	M	M	NA	Agree	0.01	4.38	0.00	21.14	7.46	51.04	NONE	POLYA	0.871972	0.073133	0.025978	0.028918	94.28	4.64	0.96	65	LP2 (2026-09-30)	PASS	NonPolyA_perM 65 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6432649	M	M	NA	Agree	0.00	3.97	0.00	22.21	8.80	52.09	NONE	POLYA	0.88188	0.093346	0.022657	0.002117	97.10	1.65	1.15	90	LP2 (2026-09-30)	PASS	NonPolyA_perM 90 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6432650	M	M	NA	Agree	0.00	4.44	0.00	25.82	10.73	60.77	NONE	POLYA	0.868244	0.102015	0.028179	0.001561	96.84	1.83	1.24	144	LP2 (2026-09-30)	PASS	NonPolyA_perM 144 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6432651	M	M	NA	Agree	0.01	4.24	0.00	24.03	10.54	56.85	NONE	POLYA	0.855222	0.115203	0.027172	0.002403	96.09	2.50	1.27	112	LP2 (2026-09-30)	PASS	NonPolyA_perM 112 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6432652	M	M	NA	Agree	0.00	3.90	0.00	20.64	9.03	47.07	NONE	POLYA	0.801459	0.079916	0.023577	0.095048	88.00	10.89	1.01	87	LP2 (2026-09-30)	PASS	NonPolyA_perM 87 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6432653	M	M	NA	Agree	0.00	3.22	0.00	19.97	8.18	55.14	NONE	POLYA	0.82829	0.05965	0.019122	0.092938	88.63	10.24	1.06	50	LP2 (2026-09-30)	PASS	NonPolyA_perM 50 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6432654	M	M	NA	Agree	0.00	3.73	0.00	22.49	7.34	55.01	NONE	POLYA	0.863808	0.077982	0.025034	0.033177	93.77	5.16	0.95	121	LP2 (2026-09-30)	PASS	NonPolyA_perM 121 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6432655	M	M	NA	Agree	0.00	3.92	0.00	23.46	8.48	48.73	NONE	POLYA	0.746075	0.088756	0.020849	0.14432	82.93	15.87	1.06	88	LP2 (2026-09-30)	PASS	NonPolyA_perM 88 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6432656	M	M	NA	Agree	0.02	4.88	0.00	26.10	10.91	62.93	NONE	POLYA	0.855542	0.113348	0.029583	0.001527	96.67	2.01	1.19	116	LP2 (2026-09-30)	PASS	NonPolyA_perM 116 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6432657	M	M	NA	Agree	0.00	4.90	0.00	26.86	11.22	61.07	NONE	POLYA	0.842258	0.127189	0.028314	0.002238	96.74	1.84	1.29	118	LP2 (2026-09-30)	PASS	NonPolyA_perM 118 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6432658	M	M	NA	Agree	0.00	3.54	0.00	20.19	8.89	55.90	NONE	POLYA	0.859378	0.111965	0.025932	0.002725	96.00	2.59	1.27	116	LP2 (2026-09-30)	PASS	NonPolyA_perM 116 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6432659	M	M	NA	Agree	0.01	4.65	0.00	25.40	9.05	64.70	NONE	POLYA	0.840087	0.078596	0.024702	0.056615	91.95	7.10	0.86	54	LP2 (2026-09-30)	PASS	NonPolyA_perM 54 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6432660	M	M	NA	Agree	0.00	2.15	0.00	11.80	5.42	42.07	NONE	POLYA	0.884862	0.06026	0.022014	0.032864	94.16	4.67	1.10	66	LP2 (2026-09-30)	PASS	NonPolyA_perM 66 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6432661	M	M	NA	Agree	0.00	2.68	0.00	16.94	9.01	46.08	NONE	POLYA	0.865827	0.062782	0.020313	0.051079	92.61	6.27	1.06	63	LP2 (2026-09-30)	PASS	NonPolyA_perM 63 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6432662	M	M	NA	Agree	0.00	5.07	0.00	24.70	10.94	56.94	NONE	POLYA	0.799227	0.124073	0.040624	0.036076	93.72	4.94	1.23	167	LP2 (2026-09-30)	PASS	NonPolyA_perM 167 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6432663	M	M	NA	Agree	0.00	1.41	0.00	5.90	3.69	40.26	NONE	POLYA	0.924695	0.049977	0.022845	0.002484	96.43	2.21	1.32	106	LP2 (2026-09-30)	PASS	NonPolyA_perM 106 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6432664	M	M	NA	Agree	0.00	2.02	0.00	13.31	6.51	50.71	NONE	POLYA	0.870762	0.101018	0.024809	0.003411	97.06	1.67	1.20	93	LP2 (2026-09-30)	PASS	NonPolyA_perM 93 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6432665	M	M	NA	Agree	0.00	2.16	0.00	15.03	6.94	51.56	NONE	POLYA	0.918427	0.047821	0.022234	0.011518	95.87	3.10	0.94	67	LP2 (2026-09-30)	PASS	NonPolyA_perM 67 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6432666	M	M	NA	Agree	0.00	3.69	0.00	26.19	10.79	55.18	NONE	POLYA	0.856572	0.112402	0.029584	0.001442	97.13	1.63	1.16	119	LP2 (2026-09-30)	PASS	NonPolyA_perM 119 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6432667	M	M	NA	Agree	0.00	4.74	0.00	37.36	7.60	67.84	NONE	POLYA	0.838858	0.132027	0.026824	0.002291	95.71	2.92	1.22	133	LP2 (2026-09-30)	PASS	NonPolyA_perM 133 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6432668	M	M	NA	Agree	0.01	3.91	0.00	24.42	9.07	52.81	NONE	POLYA	0.773273	0.094648	0.023652	0.108426	87.33	11.47	1.05	95	LP2 (2026-09-30)	PASS	NonPolyA_perM 95 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6432669	M	M	NA	Agree	0.00	4.25	0.00	21.93	9.93	54.07	NONE	POLYA	0.860174	0.109378	0.027836	0.002612	96.65	2.01	1.24	102	LP2 (2026-09-30)	PASS	NonPolyA_perM 102 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6432670	M	M	NA	Agree	0.01	4.46	0.00	32.98	9.35	63.98	NONE	POLYA	0.85346	0.118015	0.026816	0.001709	96.97	1.79	1.16	85	LP2 (2026-09-30)	PASS	NonPolyA_perM 85 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6432671	M	M	NA	Agree	0.00	2.96	0.00	21.43	7.79	53.60	NONE	POLYA	0.77182	0.079939	0.022649	0.125591	85.32	13.47	1.10	94	LP2 (2026-09-30)	PASS	NonPolyA_perM 94 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6432672	M	M	NA	Agree	0.00	2.57	0.00	14.54	7.93	53.63	NONE	POLYA	0.881073	0.052299	0.024644	0.041984	93.89	5.04	1.01	79	LP2 (2026-09-30)	PASS	NonPolyA_perM 79 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6432673	M	M	NA	Agree	0.00	3.75	0.00	22.03	7.04	61.36	NONE	POLYA	0.786048	0.085542	0.023526	0.104885	88.32	10.61	0.98	159	LP2 (2026-09-30)	PASS	NonPolyA_perM 159 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6432674	M	M	NA	Agree	0.00	5.22	0.00	27.89	10.20	63.63	NONE	POLYA	0.832564	0.102383	0.031559	0.033495	94.12	4.95	0.83	100	LP2 (2026-09-30)	PASS	NonPolyA_perM 100 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6432675	M	M	NA	Agree	0.00	5.15	0.00	29.85	9.94	69.69	NONE	POLYA	0.812677	0.113811	0.027825	0.045686	93.19	5.77	0.97	140	LP2 (2026-09-30)	PASS	NonPolyA_perM 140 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6432676	M	M	NA	Agree	0.01	4.08	0.00	24.79	9.06	51.17	NONE	POLYA	0.84594	0.120675	0.029479	0.003905	96.46	1.92	1.48	98	LP2 (2026-09-30)	PASS	NonPolyA_perM 98 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6432677	M	M	NA	Agree	0.00	4.96	0.00	20.12	8.43	57.36	NONE	POLYA	0.857063	0.078246	0.023991	0.0407	94.09	4.98	0.87	98	LP2 (2026-09-30)	PASS	NonPolyA_perM 98 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6432678	M	M	NA	Agree	0.01	5.68	0.00	21.79	8.12	61.24	NONE	POLYA	0.826009	0.141621	0.029226	0.003144	96.47	2.07	1.39	150	LP2 (2026-09-30)	PASS	NonPolyA_perM 150 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does