Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM6423045	not_specified	F	NA	Conflict	18.93	0.00	0.00	0.02	0.00	0.00	NONE	POLYA	0.945944	0.019396	0.019723	0.014936	90.28	3.21	6.39	27	LP2 (2026-09-30)	PASS	NonPolyA_perM 27 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6423046	not_specified	M	NA	Conflict	0.00	2.69	0.00	13.12	3.50	36.09	NONE	POLYA	0.946518	0.020475	0.018686	0.01432	88.99	3.26	7.64	42	LP2 (2026-09-30)	PASS	NonPolyA_perM 42 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6423047	not_specified	M	NA	Conflict	0.01	1.75	0.00	8.72	3.27	36.36	NONE	POLYA	0.954588	0.016205	0.017599	0.011608	88.20	3.69	7.96	46	LP2 (2026-09-30)	PASS	NonPolyA_perM 46 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6423048	not_specified	M	NA	Conflict	0.00	3.42	0.00	16.48	5.62	56.13	NONE	POLYA	0.954061	0.019515	0.022032	0.004393	89.46	2.49	7.92	39	LP2 (2026-09-30)	PASS	NonPolyA_perM 39 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6423049	not_specified	M	NA	Conflict	0.00	2.59	0.00	15.17	2.91	42.71	NONE	POLYA	0.953115	0.017187	0.017148	0.01255	88.64	3.03	8.19	26	LP2 (2026-09-30)	PASS	NonPolyA_perM 26 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6423050	not_specified	M	NA	Conflict	0.00	3.32	0.00	12.80	4.25	37.11	NONE	POLYA	0.951601	0.01641	0.017671	0.014319	88.47	3.60	7.80	26	LP2 (2026-09-30)	PASS	NonPolyA_perM 26 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does