Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM6340250	F	F	NA	Agree	57.50	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.862387	0.105974	0.030334	0.001304	79.69	5.17	14.70	131	LP2 (2026-09-30)	PASS	NonPolyA_perM 131 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6340251	F	F	NA	Agree	55.16	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.865369	0.098303	0.030192	0.006136	82.05	5.04	12.57	137	LP2 (2026-09-30)	PASS	NonPolyA_perM 137 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6340252	F	F	NA	Agree	105.43	0.00	0.00	0.00	0.00	0.04	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.859078	0.107194	0.031675	0.002054	81.72	4.96	12.94	150	LP2 (2026-09-30)	PASS	NonPolyA_perM 150 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6340253	F	F	NA	Agree	68.02	0.00	0.00	0.00	0.00	0.10	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.860832	0.104907	0.032745	0.001516	79.92	4.64	15.04	152	LP2 (2026-09-30)	PASS	NonPolyA_perM 152 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6340254	F	F	NA	Agree	82.59	0.03	0.00	0.00	0.00	0.05	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.862011	0.105697	0.03015	0.002142	79.94	5.08	14.64	162	LP2 (2026-09-30)	PASS	NonPolyA_perM 162 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6340255	F	F	NA	Agree	68.91	0.00	0.00	0.00	0.00	0.05	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.861511	0.106092	0.031032	0.001365	80.56	4.41	14.69	147	LP2 (2026-09-30)	PASS	NonPolyA_perM 147 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6340256	F	F	NA	Agree	79.31	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.858269	0.108803	0.031127	0.001802	82.39	4.02	13.24	156	LP2 (2026-09-30)	PASS	NonPolyA_perM 156 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6340257	F	F	NA	Agree	97.46	0.00	0.00	0.00	0.02	0.09	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.851982	0.111719	0.032634	0.003666	82.34	5.01	12.31	150	LP2 (2026-09-30)	PASS	NonPolyA_perM 150 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6340258	F	F	NA	Agree	69.84	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.843183	0.104527	0.033949	0.01834	78.95	5.84	14.84	160	LP2 (2026-09-30)	PASS	NonPolyA_perM 160 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does