Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM6260379	M	M	NA	Agree	0.00	3.26	0.00	16.96	7.14	43.34	NONE	POLYA	0.934055	0.03571	0.024761	0.005473	96.38	2.33	1.12	46	LP2 (2026-09-30)	PASS	NonPolyA_perM 46 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6260380	M	M	NA	Agree	0.00	2.74	0.00	28.18	6.67	45.69	NONE	POLYA	0.934742	0.039274	0.024234	0.001749	96.55	2.05	1.23	52	LP2 (2026-09-30)	PASS	NonPolyA_perM 52 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6260381	M	M	NA	Agree	0.00	2.99	0.00	23.82	7.29	50.73	NONE	POLYA	0.937705	0.036054	0.022438	0.003802	96.66	2.07	1.07	50	LP2 (2026-09-30)	PASS	NonPolyA_perM 50 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6260382	M	M	NA	Agree	0.01	2.88	0.00	24.18	6.62	49.40	NONE	POLYA	0.924474	0.043803	0.025003	0.00672	95.58	3.04	1.22	107	LP2 (2026-09-30)	PASS	NonPolyA_perM 107 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6260383	M	M	NA	Agree	1.95	3.74	0.00	19.95	8.60	53.29	NONE	POLYA	0.865634	0.055868	0.046925	0.031573	93.34	5.09	1.34	85	LP2 (2026-09-30)	PASS	NonPolyA_perM 85 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6260384	M	F	NA	Conflict	92.39	0.00	0.00	0.04	0.00	0.36	NONE	POLYA	0.883284	0.060361	0.027558	0.028797	93.92	4.46	1.46	95	LP2 (2026-09-30)	PASS	NonPolyA_perM 95 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6260385	M	M	NA	Agree	0.00	5.81	0.00	30.80	12.96	68.69	NONE	POLYA	0.918066	0.04544	0.031204	0.00529	96.71	1.93	1.20	113	LP2 (2026-09-30)	PASS	NonPolyA_perM 113 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does