Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM6215052	M	M	NA	Agree	0.06	18.15	0.00	62.10	17.99	106.14	NONE	POLYA	0.881367	0.078644	0.038709	0.001279	90.20	2.02	7.47	120	LP2 (2026-09-30)	PASS	NonPolyA_perM 120 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6215053	M	M	NA	Agree	0.08	13.64	0.00	55.79	17.34	96.53	NONE	POLYA	0.868671	0.084505	0.044266	0.002558	91.08	2.08	6.52	144	LP2 (2026-09-30)	PASS	NonPolyA_perM 144 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6215054	M	M	NA	Agree	0.03	11.26	0.00	55.16	13.32	79.48	NONE	POLYA	0.895207	0.066139	0.036089	0.002564	90.49	2.44	6.83	144	LP2 (2026-09-30)	PASS	NonPolyA_perM 144 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6215055	M	M	NA	Agree	0.15	11.00	0.00	48.45	15.70	74.31	NONE	POLYA	0.889353	0.070998	0.037848	0.001801	94.39	1.84	3.50	108	LP2 (2026-09-30)	PASS	NonPolyA_perM 108 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6215056	M	M	NA	Agree	0.08	6.16	0.00	33.00	11.44	84.50	NONE	POLYA	0.879307	0.073473	0.036704	0.010516	92.30	2.55	4.80	111	LP2 (2026-09-30)	PASS	NonPolyA_perM 111 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6215057	M	M	NA	Agree	0.01	7.70	0.00	41.76	11.33	68.30	NONE	POLYA	0.894434	0.067694	0.035252	0.00262	93.22	2.01	4.55	124	LP2 (2026-09-30)	PASS	NonPolyA_perM 124 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does