Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM6166111	M	M	NA	Agree	0.02	11.41	0.00	42.98	20.55	57.56	NONE	POLYA	0.915258	0.037635	0.035303	0.011804	90.97	2.64	6.19	51	LP2 (2026-09-30)	PASS	NonPolyA_perM 51 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6166112	M	M	NA	Agree	0.01	11.33	0.00	45.07	21.11	49.14	NONE	POLYA	0.883611	0.054273	0.035636	0.02648	89.26	3.95	6.58	79	LP2 (2026-09-30)	PASS	NonPolyA_perM 79 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6166113	M	M	NA	Agree	0.02	10.29	0.00	36.82	20.21	51.74	NONE	POLYA	0.912454	0.038906	0.036018	0.012622	89.98	2.88	6.91	53	LP2 (2026-09-30)	PASS	NonPolyA_perM 53 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6166114	M	M	NA	Agree	0.00	10.14	0.00	40.62	17.08	53.26	NONE	POLYA	0.90678	0.03796	0.035718	0.019542	89.43	3.41	6.94	60	LP2 (2026-09-30)	PASS	NonPolyA_perM 60 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6166115	M	M	NA	Agree	0.01	8.14	0.00	38.74	20.84	48.75	NONE	POLYA	0.852814	0.067807	0.038735	0.040644	87.34	5.41	7.02	92	LP2 (2026-09-30)	PASS	NonPolyA_perM 92 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6166116	M	M	NA	Agree	0.01	9.14	0.00	39.29	20.14	50.34	NONE	POLYA	0.894599	0.049215	0.036641	0.019545	89.29	3.33	7.16	64	LP2 (2026-09-30)	PASS	NonPolyA_perM 64 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6166117	M	M	NA	Agree	0.00	7.22	0.00	34.56	15.87	41.51	NONE	POLYA	0.834851	0.055342	0.038881	0.070926	85.82	8.52	5.38	87	LP2 (2026-09-30)	PASS	NonPolyA_perM 87 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6166118	M	M	NA	Agree	0.01	8.46	0.00	34.17	14.99	36.21	NONE	POLYA	0.868326	0.080155	0.043536	0.007983	89.62	3.15	6.90	130	LP2 (2026-09-30)	PASS	NonPolyA_perM 130 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6166119	M	M	NA	Agree	0.01	8.13	0.00	28.49	14.31	44.64	NONE	POLYA	0.877873	0.038037	0.040279	0.043811	87.21	6.08	6.42	47	LP2 (2026-09-30)	PASS	NonPolyA_perM 47 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6166120	M	M	NA	Agree	0.01	8.33	0.00	30.52	16.88	46.37	NONE	POLYA	0.878525	0.048202	0.038575	0.034698	87.72	5.04	6.98	69	LP2 (2026-09-30)	PASS	NonPolyA_perM 69 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6166121	M	M	NA	Agree	0.01	7.17	0.00	30.60	16.08	50.27	NONE	POLYA	0.899623	0.03637	0.036315	0.027691	88.80	4.34	6.61	36	LP2 (2026-09-30)	PASS	NonPolyA_perM 36 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6166122	M	M	NA	Agree	0.05	9.79	0.00	52.94	20.91	39.98	NONE	POLYA	0.820376	0.127231	0.043374	0.009019	89.12	3.09	7.45	169	LP2 (2026-09-30)	PASS	NonPolyA_perM 169 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6166123	M	M	NA	Agree	0.01	8.98	0.00	35.11	19.49	46.62	NONE	POLYA	0.84919	0.043604	0.036139	0.071066	84.00	8.11	7.69	56	LP2 (2026-09-30)	PASS	NonPolyA_perM 56 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6166124	M	M	NA	Agree	0.00	10.56	0.00	34.02	17.99	51.45	NONE	POLYA	0.865371	0.040363	0.032968	0.061299	85.10	6.86	7.83	70	LP2 (2026-09-30)	PASS	NonPolyA_perM 70 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6166125	M	M	NA	Agree	0.00	8.70	0.00	35.07	17.52	51.77	NONE	POLYA	0.882096	0.042167	0.034791	0.040946	86.71	5.25	7.81	56	LP2 (2026-09-30)	PASS	NonPolyA_perM 56 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6166126	M	M	NA	Agree	0.01	7.29	0.00	30.73	15.13	56.61	NONE	POLYA	0.911108	0.044407	0.038752	0.005733	89.33	2.66	7.78	79	LP2 (2026-09-30)	PASS	NonPolyA_perM 79 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6166127	M	M	NA	Agree	0.01	7.55	0.00	32.22	16.10	44.20	NONE	POLYA	0.815353	0.054163	0.035128	0.095355	82.56	10.26	6.98	99	LP2 (2026-09-30)	PASS	NonPolyA_perM 99 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6166128	M	M	NA	Agree	0.00	8.99	0.00	31.58	16.55	45.42	NONE	POLYA	0.891732	0.048118	0.035518	0.024632	88.64	3.90	7.26	80	LP2 (2026-09-30)	PASS	NonPolyA_perM 80 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6166129	M	M	NA	Agree	0.01	7.50	0.00	31.30	13.22	50.61	NONE	POLYA	0.913795	0.041661	0.033553	0.010991	90.26	2.65	6.89	61	LP2 (2026-09-30)	PASS	NonPolyA_perM 61 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6166130	M	M	NA	Agree	0.01	9.11	0.00	31.51	17.34	45.05	NONE	POLYA	0.884696	0.042158	0.037293	0.035853	87.93	5.01	6.81	39	LP2 (2026-09-30)	PASS	NonPolyA_perM 39 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6166131	M	M	NA	Agree	0.07	8.53	0.00	31.20	16.63	45.54	NONE	POLYA	0.850569	0.049981	0.032888	0.066562	85.42	7.72	6.64	59	LP2 (2026-09-30)	PASS	NonPolyA_perM 59 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6166132	M	M	NA	Agree	0.00	8.33	0.00	35.79	20.20	51.32	NONE	POLYA	0.886656	0.047376	0.034198	0.03177	87.71	4.51	7.53	56	LP2 (2026-09-30)	PASS	NonPolyA_perM 56 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6166133	M	M	NA	Agree	0.00	7.51	0.00	30.54	14.97	49.37	NONE	POLYA	0.893377	0.036524	0.036339	0.03376	88.42	4.75	6.61	51	LP2 (2026-09-30)	PASS	NonPolyA_perM 51 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6166134	M	M	NA	Agree	0.01	8.50	0.00	31.46	17.58	41.48	NONE	POLYA	0.861415	0.053752	0.036051	0.048782	87.50	6.21	6.05	70	LP2 (2026-09-30)	PASS	NonPolyA_perM 70 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does