Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM6070047	M	M	NA	Agree	0.00	5.48	0.00	17.66	5.75	48.60	NONE	POLYA	0.952514	0.016593	0.024909	0.005984	96.01	2.11	1.75	16	LP2 (2026-09-30)	PASS	NonPolyA_perM 16 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6070048	M	M	NA	Agree	0.00	5.74	0.00	30.13	8.48	58.36	NONE	POLYA	0.94603	0.020889	0.028534	0.004547	95.98	2.03	1.85	27	LP2 (2026-09-30)	PASS	NonPolyA_perM 27 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6070049	M	M	NA	Agree	0.00	5.68	0.00	22.99	7.44	65.31	NONE	POLYA	0.948443	0.018447	0.024825	0.008285	95.76	2.27	1.85	28	LP2 (2026-09-30)	PASS	NonPolyA_perM 28 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6070050	M	M	NA	Agree	0.00	5.01	0.00	21.76	7.66	64.27	NONE	POLYA	0.94533	0.018439	0.025536	0.010695	95.22	2.57	2.08	21	LP2 (2026-09-30)	PASS	NonPolyA_perM 21 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6070051	M	M	NA	Agree	0.00	4.37	0.00	16.58	6.05	46.28	NONE	POLYA	0.953019	0.018602	0.025424	0.002955	96.37	1.79	1.72	22	LP2 (2026-09-30)	PASS	NonPolyA_perM 22 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6070052	M	M	NA	Agree	0.00	5.07	0.00	18.99	11.25	51.61	NONE	POLYA	0.94478	0.025184	0.026481	0.003555	95.90	1.85	2.12	36	LP2 (2026-09-30)	PASS	NonPolyA_perM 36 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6070053	M	M	NA	Agree	0.00	4.11	0.00	15.36	6.89	43.57	NONE	POLYA	0.942836	0.024768	0.025575	0.006821	95.37	2.25	2.22	31	LP2 (2026-09-30)	PASS	NonPolyA_perM 31 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6070054	M	M	NA	Agree	0.00	4.52	0.00	17.87	5.70	49.61	NONE	POLYA	0.946735	0.022348	0.022532	0.008385	95.36	2.14	2.37	26	LP2 (2026-09-30)	PASS	NonPolyA_perM 26 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6070055	M	M	NA	Agree	0.00	10.29	0.00	35.72	10.16	62.49	NONE	POLYA	0.928275	0.023126	0.026535	0.022064	93.39	3.36	3.15	24	LP2 (2026-09-30)	PASS	NonPolyA_perM 24 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6070056	M	M	NA	Agree	0.00	9.38	0.00	35.19	9.97	63.25	NONE	POLYA	0.947425	0.022127	0.026643	0.003805	95.31	1.73	2.85	21	LP2 (2026-09-30)	PASS	NonPolyA_perM 21 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6070057	M	M	NA	Agree	0.00	8.82	0.00	30.65	8.95	59.34	NONE	POLYA	0.919273	0.017541	0.02596	0.037226	91.26	4.73	3.91	25	LP2 (2026-09-30)	PASS	NonPolyA_perM 25 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6070058	M	M	NA	Agree	0.00	9.00	0.00	31.87	9.74	61.49	NONE	POLYA	0.93023	0.021219	0.026134	0.022418	93.43	3.50	2.95	13	LP2 (2026-09-30)	PASS	NonPolyA_perM 13 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6070059	M	M	NA	Agree	0.00	3.63	0.00	19.34	4.37	46.97	NONE	POLYA	0.961149	0.014843	0.020998	0.00301	96.24	1.78	1.85	10	LP2 (2026-09-30)	PASS	NonPolyA_perM 10 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6070060	M	M	NA	Agree	0.00	4.08	0.00	13.28	4.38	39.65	NONE	POLYA	0.967271	0.01189	0.017705	0.003134	95.80	1.63	2.49	13	LP2 (2026-09-30)	PASS	NonPolyA_perM 13 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6070061	M	M	NA	Agree	0.00	2.41	0.00	6.20	3.53	22.09	NONE	POLYA	0.962471	0.012006	0.017933	0.00759	94.59	2.10	3.20	10	LP2 (2026-09-30)	PASS	NonPolyA_perM 10 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6070062	M	M	NA	Agree	0.00	3.31	0.00	15.60	5.50	46.91	NONE	POLYA	0.953289	0.016881	0.020913	0.008917	95.15	2.31	2.41	24	LP2 (2026-09-30)	PASS	NonPolyA_perM 24 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6070063	M	M	NA	Agree	0.00	5.42	0.00	30.74	6.57	61.21	NONE	POLYA	0.958523	0.013388	0.02026	0.00783	94.83	2.14	2.94	15	LP2 (2026-09-30)	PASS	NonPolyA_perM 15 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6070064	M	M	NA	Agree	0.00	5.85	0.00	21.91	5.94	52.86	NONE	POLYA	0.959072	0.012899	0.020035	0.007994	94.47	2.25	3.19	8	LP2 (2026-09-30)	PASS	NonPolyA_perM 8 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6070065	M	M	NA	Agree	0.00	5.42	0.00	21.19	7.65	48.15	NONE	POLYA	0.95248	0.019511	0.024579	0.003431	96.14	1.83	1.90	19	LP2 (2026-09-30)	PASS	NonPolyA_perM 19 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6070066	M	M	NA	Agree	0.00	5.23	0.00	19.53	5.51	47.53	NONE	POLYA	0.951681	0.019506	0.024068	0.004744	96.30	1.88	1.72	24	LP2 (2026-09-30)	PASS	NonPolyA_perM 24 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6070067	M	M	NA	Agree	0.01	3.85	0.00	13.54	4.84	42.11	NONE	POLYA	0.953408	0.018295	0.022366	0.005931	95.52	2.01	2.34	26	LP2 (2026-09-30)	PASS	NonPolyA_perM 26 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6070068	M	M	NA	Agree	0.00	3.06	0.00	12.66	4.97	37.62	NONE	POLYA	0.952094	0.018384	0.021886	0.007636	94.99	2.20	2.67	23	LP2 (2026-09-30)	PASS	NonPolyA_perM 23 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6070069	M	M	NA	Agree	0.00	9.27	0.00	31.37	8.43	53.21	NONE	POLYA	0.947601	0.021331	0.026749	0.004319	95.10	1.83	2.95	27	LP2 (2026-09-30)	PASS	NonPolyA_perM 27 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6070070	M	M	NA	Agree	0.01	9.28	0.00	36.84	9.59	60.00	NONE	POLYA	0.939503	0.02649	0.028	0.006006	95.03	2.00	2.85	24	LP2 (2026-09-30)	PASS	NonPolyA_perM 24 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6070071	M	M	NA	Agree	0.00	8.44	0.00	29.97	8.41	57.37	NONE	POLYA	0.950666	0.018486	0.027209	0.003638	95.12	1.80	2.95	19	LP2 (2026-09-30)	PASS	NonPolyA_perM 19 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6070072	M	M	NA	Agree	0.00	9.42	0.00	32.65	9.61	58.38	NONE	POLYA	0.947116	0.019738	0.027106	0.00604	94.93	2.01	2.93	14	LP2 (2026-09-30)	PASS	NonPolyA_perM 14 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6070073	M	M	NA	Agree	0.00	11.88	0.00	33.45	9.40	64.28	NONE	POLYA	0.944492	0.022552	0.027088	0.005868	94.68	1.92	3.29	27	LP2 (2026-09-30)	PASS	NonPolyA_perM 27 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6070074	M	M	NA	Agree	0.00	8.50	0.00	37.89	8.67	70.29	NONE	POLYA	0.951849	0.017804	0.024359	0.005988	94.79	1.95	3.14	15	LP2 (2026-09-30)	PASS	NonPolyA_perM 15 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6070075	M	M	NA	Agree	0.00	8.55	0.00	33.94	9.88	62.70	NONE	POLYA	0.946056	0.019134	0.028237	0.006573	94.47	2.04	3.38	23	LP2 (2026-09-30)	PASS	NonPolyA_perM 23 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6070076	M	M	NA	Agree	0.00	9.17	0.00	34.12	8.80	62.94	NONE	POLYA	0.941699	0.021631	0.028082	0.008588	94.29	2.27	3.30	20	LP2 (2026-09-30)	PASS	NonPolyA_perM 20 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6070077	M	M	NA	Agree	0.01	4.86	0.00	22.91	6.60	54.29	NONE	POLYA	0.955751	0.016583	0.024623	0.003044	96.15	1.84	1.87	19	LP2 (2026-09-30)	PASS	NonPolyA_perM 19 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6070078	M	M	NA	Agree	0.00	5.90	0.00	27.82	7.41	63.93	NONE	POLYA	0.953011	0.020879	0.021673	0.004437	96.11	1.88	1.90	25	LP2 (2026-09-30)	PASS	NonPolyA_perM 25 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6070079	M	M	NA	Agree	0.00	4.71	0.00	21.03	6.50	56.71	NONE	POLYA	0.943486	0.018487	0.022787	0.01524	94.78	2.84	2.26	24	LP2 (2026-09-30)	PASS	NonPolyA_perM 24 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6070080	M	M	NA	Agree	0.01	4.71	0.00	22.57	6.98	66.63	NONE	POLYA	0.950029	0.019608	0.021913	0.00845	95.31	2.31	2.26	23	LP2 (2026-09-30)	PASS	NonPolyA_perM 23 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6070081	M	M	NA	Agree	0.01	9.65	0.00	34.35	9.14	70.01	NONE	POLYA	0.943146	0.018116	0.02411	0.014628	93.89	2.71	3.30	19	LP2 (2026-09-30)	PASS	NonPolyA_perM 19 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6070082	M	M	NA	Agree	0.00	9.53	0.00	40.82	9.77	76.88	NONE	POLYA	0.928577	0.01724	0.019933	0.03425	92.39	4.44	3.08	13	LP2 (2026-09-30)	PASS	NonPolyA_perM 13 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6070083	M	M	NA	Agree	0.00	6.46	0.00	24.47	7.62	52.41	NONE	POLYA	0.92681	0.01487	0.024077	0.034242	91.78	4.50	3.62	28	LP2 (2026-09-30)	PASS	NonPolyA_perM 28 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6070084	M	M	NA	Agree	0.00	7.72	0.00	29.75	8.11	63.60	NONE	POLYA	0.946323	0.017917	0.02555	0.010209	94.14	2.39	3.36	16	LP2 (2026-09-30)	PASS	NonPolyA_perM 16 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does