Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM6058111	M	M	NA	Agree	0.02	6.86	0.00	38.08	15.74	68.07	NONE	POLYA	0.896916	0.05591	0.044635	0.002538	97.62	1.61	0.63	50	LP2 (2026-09-30)	PASS	NonPolyA_perM 50 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6058112	M	M	NA	Agree	0.02	7.72	0.00	35.33	16.36	71.40	NONE	POLYA	0.884049	0.068499	0.045206	0.002246	97.64	1.60	0.63	56	LP2 (2026-09-30)	PASS	NonPolyA_perM 56 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6058113	M	M	NA	Agree	0.07	7.17	0.00	35.68	17.42	73.80	NONE	POLYA	0.874411	0.077303	0.046425	0.001861	97.65	1.55	0.66	69	LP2 (2026-09-30)	PASS	NonPolyA_perM 69 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6058114	M	M	NA	Agree	0.01	7.31	0.00	47.44	15.38	76.27	NONE	POLYA	0.874937	0.073599	0.047037	0.004427	97.03	2.06	0.72	77	LP2 (2026-09-30)	PASS	NonPolyA_perM 77 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6058115	M	M	NA	Agree	0.03	6.54	0.00	32.81	10.04	73.80	NONE	POLYA	0.863403	0.085835	0.046416	0.004346	96.71	2.24	0.85	116	LP2 (2026-09-30)	PASS	NonPolyA_perM 116 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6058116	M	M	NA	Agree	0.03	6.65	0.00	39.66	15.13	70.59	NONE	POLYA	0.86301	0.087889	0.046023	0.003078	97.50	1.73	0.62	79	LP2 (2026-09-30)	PASS	NonPolyA_perM 79 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does