Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-20) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM6034521	M	M	NA	Agree	0.00	3.81	0.00	28.79	10.79	57.78	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.894576	0.066644	0.036252	0.002528	92.49	2.33	5.00	123	LP2 (2026-09-30)	PASS	NonPolyA_perM 123 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6034522	M	M	NA	Agree	0.01	4.25	0.00	30.77	8.62	53.78	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.899129	0.064008	0.033841	0.003022	92.29	2.39	5.10	130	LP2 (2026-09-30)	PASS	NonPolyA_perM 130 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6034523	M	M	NA	Agree	0.06	5.64	0.00	42.26	12.71	56.33	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.889699	0.073124	0.034518	0.002659	93.94	2.14	3.74	124	LP2 (2026-09-30)	PASS	NonPolyA_perM 124 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6034524	M	M	NA	Agree	0.02	4.10	0.00	28.08	10.23	57.01	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.8935	0.068114	0.035204	0.003183	92.75	2.34	4.73	107	LP2 (2026-09-30)	PASS	NonPolyA_perM 107 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6034525	M	M	NA	Agree	0.01	4.45	0.00	21.63	9.60	53.33	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.893184	0.062799	0.040849	0.003168	90.73	2.64	6.41	123	LP2 (2026-09-30)	PASS	NonPolyA_perM 123 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6034526	M	M	NA	Agree	0.00	3.03	0.00	23.57	5.63	51.38	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.919033	0.049195	0.02747	0.004302	90.84	2.67	6.25	96	LP2 (2026-09-30)	PASS	NonPolyA_perM 96 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does