Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM6022316	M	M	NA	Agree	0.00	2.50	0.00	7.81	6.23	25.12	NONE	POLYA	0.946256	0.022823	0.027139	0.003782	89.85	2.66	7.32	26	LP2 (2026-09-30)	PASS	NonPolyA_perM 26 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6022317	M	M	NA	Agree	0.00	1.73	0.00	6.50	5.52	17.05	NONE	POLYA	0.943636	0.024813	0.026961	0.00459	89.93	2.63	7.25	32	LP2 (2026-09-30)	PASS	NonPolyA_perM 32 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6022318	M	M	NA	Agree	0.01	3.47	0.00	11.97	7.92	35.37	NONE	POLYA	0.934871	0.03027	0.031069	0.00379	90.44	2.51	6.85	38	LP2 (2026-09-30)	PASS	NonPolyA_perM 38 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6022319	M	M	NA	Agree	0.01	2.62	0.00	10.59	6.57	26.92	NONE	POLYA	0.940752	0.025201	0.02997	0.004076	89.91	2.67	7.24	31	LP2 (2026-09-30)	PASS	NonPolyA_perM 31 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6022320	M	M	NA	Agree	0.02	1.16	0.00	4.13	4.77	16.21	NONE	POLYA	0.952766	0.01767	0.025657	0.003906	89.55	2.25	8.02	28	LP2 (2026-09-30)	PASS	NonPolyA_perM 28 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6022321	M	M	NA	Agree	0.02	2.09	0.00	5.23	5.04	18.97	NONE	POLYA	0.953984	0.018243	0.024363	0.00341	89.35	2.24	8.20	18	LP2 (2026-09-30)	PASS	NonPolyA_perM 18 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6022322	M	M	NA	Agree	0.00	2.98	0.00	11.38	10.53	32.23	NONE	POLYA	0.94762	0.023735	0.024029	0.004617	89.88	2.50	7.48	31	LP2 (2026-09-30)	PASS	NonPolyA_perM 31 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6022323	M	M	NA	Agree	0.00	1.43	0.00	5.29	4.20	19.39	NONE	POLYA	0.953936	0.018571	0.023713	0.00378	90.32	2.17	7.35	20	LP2 (2026-09-30)	PASS	NonPolyA_perM 20 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6022324	M	M	NA	Agree	0.00	3.71	0.00	13.80	7.32	37.78	NONE	POLYA	0.937251	0.031199	0.027825	0.003725	89.21	2.22	8.39	36	LP2 (2026-09-30)	PASS	NonPolyA_perM 36 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6022325	M	M	NA	Agree	0.00	1.83	0.00	6.78	5.72	21.38	NONE	POLYA	0.951739	0.018465	0.024926	0.004869	89.03	2.26	8.54	23	LP2 (2026-09-30)	PASS	NonPolyA_perM 23 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6022326	M	M	NA	Agree	0.00	2.35	0.00	8.55	6.88	27.29	NONE	POLYA	0.946308	0.023876	0.025705	0.004111	89.93	2.29	7.63	20	LP2 (2026-09-30)	PASS	NonPolyA_perM 20 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6022327	M	M	NA	Agree	0.00	1.33	0.00	6.69	6.09	19.31	NONE	POLYA	0.952986	0.018327	0.024826	0.003862	88.82	2.22	8.80	22	LP2 (2026-09-30)	PASS	NonPolyA_perM 22 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6022328	M	M	NA	Agree	0.00	1.80	0.00	7.10	5.99	18.55	NONE	POLYA	0.950727	0.022148	0.023461	0.003664	90.42	2.01	7.43	39	LP2 (2026-09-30)	PASS	NonPolyA_perM 39 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6022329	M	M	NA	Agree	0.01	1.69	0.00	8.26	6.52	21.35	NONE	POLYA	0.941351	0.028889	0.026178	0.003582	90.22	2.15	7.48	38	LP2 (2026-09-30)	PASS	NonPolyA_perM 38 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6022330	M	M	NA	Agree	0.00	1.28	0.00	7.19	5.36	18.23	NONE	POLYA	0.949042	0.02089	0.026157	0.003911	89.66	2.21	7.96	25	LP2 (2026-09-30)	PASS	NonPolyA_perM 25 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM6022331	M	M	NA	Agree	0.00	2.99	0.00	13.25	7.64	30.55	NONE	POLYA	0.94288	0.026388	0.027203	0.003529	90.64	2.30	6.87	28	LP2 (2026-09-30)	PASS	NonPolyA_perM 28 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does