Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM5782155	M	M	NA	Agree	0.05	4.00	0.00	35.93	8.10	73.77	NONE	POLYA	0.913695	0.038684	0.042777	0.004843	93.90	2.03	3.95	48	LP2 (2026-09-30)	PASS	NonPolyA_perM 48 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5782156	M	M	NA	Agree	0.05	4.93	0.00	38.58	9.85	79.79	NONE	POLYA	0.908744	0.045628	0.042414	0.003214	94.34	1.92	3.59	39	LP2 (2026-09-30)	PASS	NonPolyA_perM 39 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5782157	M	M	NA	Agree	0.09	4.43	0.00	36.24	9.82	77.39	NONE	POLYA	0.904023	0.047312	0.044289	0.004375	93.65	2.00	4.21	58	LP2 (2026-09-30)	PASS	NonPolyA_perM 58 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5782158	M	M	NA	Agree	0.04	5.52	0.00	38.95	11.07	70.76	NONE	POLYA	0.90407	0.051032	0.041796	0.003101	94.48	1.83	3.55	51	LP2 (2026-09-30)	PASS	NonPolyA_perM 51 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5782159	M	M	NA	Agree	0.06	5.43	0.00	31.10	8.68	63.60	NONE	POLYA	0.898469	0.055035	0.04214	0.004356	93.77	1.99	4.09	78	LP2 (2026-09-30)	PASS	NonPolyA_perM 78 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5782160	M	M	NA	Agree	0.05	6.23	0.00	37.17	10.38	76.57	NONE	POLYA	0.899009	0.056555	0.041333	0.003102	94.23	1.79	3.83	67	LP2 (2026-09-30)	PASS	NonPolyA_perM 67 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5782161	M	M	NA	Agree	0.03	5.40	0.00	37.97	11.19	67.01	NONE	POLYA	0.911666	0.042037	0.042008	0.004288	94.40	1.84	3.63	56	LP2 (2026-09-30)	PASS	NonPolyA_perM 56 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5782162	M	M	NA	Agree	0.01	4.74	0.00	33.91	11.29	60.68	NONE	POLYA	0.898807	0.053903	0.043823	0.003467	94.09	1.79	3.99	64	LP2 (2026-09-30)	PASS	NonPolyA_perM 64 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5782163	M	M	NA	Agree	0.08	4.21	0.00	36.33	8.52	73.49	NONE	POLYA	0.899673	0.052389	0.043662	0.004275	93.51	1.90	4.45	64	LP2 (2026-09-30)	PASS	NonPolyA_perM 64 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does