Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-22) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM5767953	M	M	NA	Agree	0.17	4.77	0.00	33.14	18.27	50.07	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.843985	0.105259	0.047745	0.003011	88.41	1.75	9.65	112	LP2 (2026-09-30)	PASS	NonPolyA_perM 112 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5767954	M	M	NA	Agree	0.22	4.99	0.00	27.83	16.01	44.63	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.854182	0.097449	0.045881	0.002487	90.09	1.68	8.04	88	LP2 (2026-09-30)	PASS	NonPolyA_perM 88 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5767955	M	M	NA	Agree	0.11	3.88	0.00	25.78	12.93	45.21	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.857408	0.093997	0.045465	0.00313	87.87	1.73	10.21	83	LP2 (2026-09-30)	PASS	NonPolyA_perM 83 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5767956	M	M	NA	Agree	0.28	4.43	0.00	26.58	16.94	45.80	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.848256	0.102882	0.046028	0.002834	89.45	1.69	8.68	104	LP2 (2026-09-30)	PASS	NonPolyA_perM 104 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5767957	M	M	NA	Agree	0.07	4.15	0.00	28.86	16.81	42.17	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.852871	0.098559	0.046195	0.002375	89.18	1.68	8.95	87	LP2 (2026-09-30)	PASS	NonPolyA_perM 87 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5767958	M	M	NA	Agree	0.23	4.73	0.00	32.43	17.64	50.05	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.840139	0.108877	0.04768	0.003304	86.52	1.81	11.46	97	LP2 (2026-09-30)	PASS	NonPolyA_perM 97 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5767959	M	M	NA	Agree	0.24	4.96	0.00	29.16	13.42	46.49	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.832605	0.09151	0.046237	0.029648	85.00	4.37	10.44	91	LP2 (2026-09-30)	PASS	NonPolyA_perM 91 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5767960	M	M	NA	Agree	0.17	3.29	0.00	32.39	13.77	50.55	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.847604	0.101057	0.046882	0.004457	87.67	1.90	10.23	109	LP2 (2026-09-30)	PASS	NonPolyA_perM 109 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5767961	M	M	NA	Agree	0.14	5.10	0.00	30.42	15.64	49.79	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.843627	0.104203	0.04816	0.004009	86.35	1.87	11.58	91	LP2 (2026-09-30)	PASS	NonPolyA_perM 91 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5767962	M	M	NA	Agree	0.12	5.68	0.00	33.89	14.88	49.66	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.812728	0.106644	0.046975	0.033653	84.36	4.74	10.70	115	LP2 (2026-09-30)	PASS	NonPolyA_perM 115 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does