Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM5704631	M	M	NA	Agree	0.00	4.32	0.00	32.45	13.88	63.61	NONE	POLYA	0.836627	0.097014	0.045321	0.021038	93.74	3.47	2.57	88	LP2 (2026-09-30)	PASS	NonPolyA_perM 88 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5704632	M	M	NA	Agree	0.01	6.55	0.00	43.05	15.88	69.48	NONE	POLYA	0.807298	0.13037	0.046683	0.015649	94.39	2.91	2.45	104	LP2 (2026-09-30)	PASS	NonPolyA_perM 104 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5704633	M	M	NA	Agree	0.00	6.62	0.00	37.17	14.67	64.52	NONE	POLYA	0.79968	0.130536	0.045006	0.024779	93.54	3.83	2.41	133	LP2 (2026-09-30)	PASS	NonPolyA_perM 133 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5704634	M	M	NA	Agree	0.00	4.10	0.00	28.99	8.89	64.08	NONE	POLYA	0.720028	0.106326	0.040132	0.133513	82.80	14.16	2.83	103	LP2 (2026-09-30)	PASS	NonPolyA_perM 103 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5704635	M	M	NA	Agree	0.00	5.77	0.00	37.78	12.87	69.30	NONE	POLYA	0.818843	0.116167	0.045786	0.019204	93.78	3.26	2.73	108	LP2 (2026-09-30)	PASS	NonPolyA_perM 108 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5704636	M	M	NA	Agree	0.00	6.43	0.00	34.35	12.28	68.67	NONE	POLYA	0.793742	0.124805	0.045398	0.036054	92.22	4.89	2.66	118	LP2 (2026-09-30)	PASS	NonPolyA_perM 118 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5704637	M	M	NA	Agree	0.00	5.50	0.00	40.45	12.65	72.46	NONE	POLYA	0.799548	0.122118	0.044509	0.033825	92.60	4.63	2.54	108	LP2 (2026-09-30)	PASS	NonPolyA_perM 108 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5704638	M	M	NA	Agree	0.00	4.61	0.00	36.81	11.73	67.55	NONE	POLYA	0.803579	0.121228	0.046911	0.028282	92.91	4.18	2.69	120	LP2 (2026-09-30)	PASS	NonPolyA_perM 120 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does