Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-22) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM5555810	M	M	NA	Agree	0.02	4.52	0.00	23.29	14.42	49.15	NONE	POLYA	0.887005	0.059284	0.03961	0.014101	92.95	3.12	3.71	133	LP2 (2026-09-30)	PASS	NonPolyA_perM 133 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5555811	M	M	NA	Agree	0.01	4.94	0.00	28.61	17.20	50.43	NONE	POLYA	0.881439	0.062386	0.041564	0.014611	92.32	3.38	4.11	102	LP2 (2026-09-30)	PASS	NonPolyA_perM 102 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5555812	M	M	NA	Agree	0.02	4.52	0.00	24.33	22.28	58.86	NONE	POLYA	0.878045	0.065299	0.04263	0.014026	92.37	3.36	4.01	101	LP2 (2026-09-30)	PASS	NonPolyA_perM 101 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5555813	M	M	NA	Agree	0.04	3.31	0.00	21.51	15.14	54.19	NONE	POLYA	0.880725	0.054865	0.038075	0.026335	91.42	4.36	4.03	87	LP2 (2026-09-30)	PASS	NonPolyA_perM 87 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5555814	M	M	NA	Agree	0.00	3.21	0.00	19.67	15.13	50.91	NONE	POLYA	0.895713	0.043951	0.040988	0.019348	91.35	3.80	4.68	99	LP2 (2026-09-30)	PASS	NonPolyA_perM 99 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5555815	M	M	NA	Agree	0.00	3.04	0.00	16.33	9.49	45.46	NONE	POLYA	0.888577	0.046707	0.03925	0.025466	91.61	4.24	3.96	78	LP2 (2026-09-30)	PASS	NonPolyA_perM 78 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5555816	M	M	NA	Agree	0.02	3.91	0.00	19.45	14.24	48.58	NONE	POLYA	0.895226	0.04809	0.039803	0.016882	92.05	3.65	4.06	86	LP2 (2026-09-30)	PASS	NonPolyA_perM 86 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5555817	M	M	NA	Agree	0.00	3.76	0.00	21.27	18.68	50.93	NONE	POLYA	0.885208	0.059728	0.041526	0.013539	92.57	3.46	3.69	89	LP2 (2026-09-30)	PASS	NonPolyA_perM 89 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5555818	M	M	NA	Agree	0.00	4.75	0.00	22.02	18.68	57.11	NONE	POLYA	0.876084	0.066086	0.044078	0.013752	91.92	3.31	4.52	119	LP2 (2026-09-30)	PASS	NonPolyA_perM 119 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5555819	M	M	NA	Agree	0.00	4.76	0.00	24.88	15.11	54.80	NONE	POLYA	0.883309	0.059374	0.039371	0.017946	92.54	3.47	3.79	118	LP2 (2026-09-30)	PASS	NonPolyA_perM 118 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5555820	M	M	NA	Agree	0.01	3.86	0.00	21.62	11.63	45.17	NONE	POLYA	0.896899	0.047821	0.039178	0.016101	92.79	3.40	3.60	54	LP2 (2026-09-30)	PASS	NonPolyA_perM 54 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5555821	M	M	NA	Agree	0.00	3.77	0.00	23.07	16.97	53.07	NONE	POLYA	0.880913	0.06215	0.042297	0.01464	92.53	3.36	3.90	88	LP2 (2026-09-30)	PASS	NonPolyA_perM 88 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does