Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM5466833	not_specified	M	NA	Conflict	0.00	8.85	0.00	44.63	11.34	116.11	NONE	POLYA	0.893187	0.066585	0.03724	0.002988	92.94	2.57	4.33	95	LP2 (2026-09-30)	PASS	NonPolyA_perM 95 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5466834	not_specified	F	NA	Conflict	166.92	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.91822	0.052265	0.027318	0.002197	95.15	2.06	2.65	67	LP2 (2026-09-30)	PASS	NonPolyA_perM 67 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5466835	not_specified	F	NA	Conflict	144.00	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.925002	0.045027	0.026554	0.003417	95.00	2.21	2.66	58	LP2 (2026-09-30)	PASS	NonPolyA_perM 58 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5466836	not_specified	F	NA	Conflict	225.17	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.908799	0.061027	0.029189	0.000986	94.00	1.87	3.96	63	LP2 (2026-09-30)	PASS	NonPolyA_perM 63 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5466837	not_specified	F	NA	Conflict	107.58	0.02	0.00	0.00	0.00	0.00	NONE	POLYA	0.909547	0.049383	0.026951	0.014119	93.49	3.24	3.12	67	LP2 (2026-09-30)	PASS	NonPolyA_perM 67 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5466838	not_specified	F	NA	Conflict	93.49	0.00	0.00	0.02	0.00	0.00	NONE	POLYA	0.91748	0.047266	0.023506	0.011749	94.06	2.85	2.91	59	LP2 (2026-09-30)	PASS	NonPolyA_perM 59 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does