Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-22) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM5440790	M	M	NA	Agree	0.04	7.96	0.00	45.29	15.86	46.47	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.864879	0.085973	0.043979	0.005169	96.08	2.06	1.59	61	LP2 (2026-09-30)	PASS	NonPolyA_perM 61 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5440791	M	M	NA	Agree	0.01	8.17	0.00	43.69	16.12	54.69	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.903857	0.063204	0.028033	0.004906	96.69	1.70	1.41	42	LP2 (2026-09-30)	PASS	NonPolyA_perM 42 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5440792	M	M	NA	Agree	0.04	8.95	0.00	45.47	17.78	55.41	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.885693	0.074621	0.03119	0.008495	96.02	2.17	1.58	59	LP2 (2026-09-30)	PASS	NonPolyA_perM 59 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5440793	M	M	NA	Agree	0.07	8.53	0.00	48.75	19.27	48.58	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.853098	0.10034	0.041555	0.005007	95.89	2.14	1.67	85	LP2 (2026-09-30)	PASS	NonPolyA_perM 85 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5440794	M	M	NA	Agree	0.08	7.14	0.00	46.25	22.38	54.37	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.850801	0.098854	0.047108	0.003237	96.16	2.03	1.48	121	LP2 (2026-09-30)	PASS	NonPolyA_perM 121 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5440795	M	M	NA	Agree	0.01	7.33	0.00	46.95	18.08	46.11	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.858184	0.094658	0.044468	0.00269	96.24	1.92	1.54	85	LP2 (2026-09-30)	PASS	NonPolyA_perM 85 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5440796	M	M	NA	Agree	0.09	8.18	0.00	50.59	19.83	47.46	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.846527	0.104284	0.045611	0.003579	96.15	2.00	1.59	105	LP2 (2026-09-30)	PASS	NonPolyA_perM 105 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5440797	M	M	NA	Agree	0.02	8.44	0.00	44.28	15.16	47.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.87045	0.084149	0.041356	0.004046	96.11	2.09	1.54	79	LP2 (2026-09-30)	PASS	NonPolyA_perM 79 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5440798	M	M	NA	Agree	0.08	7.27	0.00	38.99	16.31	49.79	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.87095	0.078548	0.04047	0.010033	95.51	2.73	1.50	97	LP2 (2026-09-30)	PASS	NonPolyA_perM 97 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5440799	M	M	NA	Agree	0.09	8.05	0.00	50.18	18.79	51.48	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.880338	0.079071	0.036036	0.004554	95.90	1.94	1.93	68	LP2 (2026-09-30)	PASS	NonPolyA_perM 68 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5440800	M	M	NA	Agree	0.06	9.28	0.00	54.07	19.97	46.45	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.866971	0.085039	0.044452	0.003538	96.42	2.04	1.30	85	LP2 (2026-09-30)	PASS	NonPolyA_perM 85 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5440801	M	M	NA	Agree	0.08	7.26	0.00	47.63	22.99	43.47	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.843958	0.100889	0.050308	0.004845	96.27	2.07	1.40	100	LP2 (2026-09-30)	PASS	NonPolyA_perM 100 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5440802	M	M	NA	Agree	0.03	9.59	0.00	63.31	19.43	51.77	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.881402	0.07863	0.036361	0.003606	96.25	1.88	1.63	67	LP2 (2026-09-30)	PASS	NonPolyA_perM 67 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5440803	M	M	NA	Agree	0.05	8.81	0.00	49.49	18.47	54.02	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.870018	0.084808	0.039289	0.005884	95.69	2.25	1.79	75	LP2 (2026-09-30)	PASS	NonPolyA_perM 75 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5440804	M	M	NA	Agree	0.05	7.37	0.00	44.70	18.00	48.21	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.867513	0.088409	0.04012	0.003958	96.42	1.94	1.38	82	LP2 (2026-09-30)	PASS	NonPolyA_perM 82 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5440805	M	M	NA	Agree	0.01	8.10	0.00	52.25	20.77	48.40	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.854603	0.09427	0.048456	0.002671	96.48	1.91	1.32	95	LP2 (2026-09-30)	PASS	NonPolyA_perM 95 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does