Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-20) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM5334786	M	M	NA	Agree	0.02	7.82	0.00	60.53	16.03	69.42	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.893751	0.062404	0.040938	0.002907	92.84	2.70	4.14	65	LP2 (2026-09-30)	PASS	NonPolyA_perM 65 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5334787	M	M	NA	Agree	0.04	6.87	0.00	63.42	17.25	72.53	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.890114	0.065761	0.041404	0.00272	93.19	2.73	3.77	68	LP2 (2026-09-30)	PASS	NonPolyA_perM 68 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5334788	M	M	NA	Agree	0.04	8.14	0.00	61.61	17.61	67.39	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.886288	0.06837	0.041575	0.003767	93.01	2.82	3.84	62	LP2 (2026-09-30)	PASS	NonPolyA_perM 62 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5334789	M	M	NA	Agree	0.02	8.10	0.00	61.45	17.44	72.58	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.891621	0.063884	0.042048	0.002447	93.70	2.82	3.15	62	LP2 (2026-09-30)	PASS	NonPolyA_perM 62 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5334790	M	M	NA	Agree	0.01	7.90	0.00	65.23	18.07	73.97	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.893388	0.062133	0.041779	0.0027	93.34	2.74	3.62	64	LP2 (2026-09-30)	PASS	NonPolyA_perM 64 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5334791	M	M	NA	Agree	0.02	8.27	0.00	65.81	17.29	71.79	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.888545	0.067302	0.041493	0.00266	93.22	2.66	3.80	76	LP2 (2026-09-30)	PASS	NonPolyA_perM 76 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5334792	M	M	NA	Agree	0.02	6.21	0.00	47.04	12.63	57.45	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.890358	0.066248	0.040173	0.003221	92.59	2.79	4.28	58	LP2 (2026-09-30)	PASS	NonPolyA_perM 58 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5334793	M	M	NA	Agree	0.03	7.82	0.00	60.57	16.93	67.53	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.893934	0.062449	0.040622	0.002995	93.34	2.77	3.57	72	LP2 (2026-09-30)	PASS	NonPolyA_perM 72 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5334794	M	M	NA	Agree	0.04	7.21	0.00	58.51	16.98	68.47	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.891186	0.063521	0.041723	0.003571	93.08	2.73	3.86	70	LP2 (2026-09-30)	PASS	NonPolyA_perM 70 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5334795	M	M	NA	Agree	0.04	8.29	0.00	63.90	16.52	70.50	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.891767	0.063337	0.041638	0.003258	93.22	2.72	3.74	67	LP2 (2026-09-30)	PASS	NonPolyA_perM 67 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does