Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM5211757	M	M	NA	Agree	0.00	2.85	0.00	14.24	3.67	38.77	NONE	POLYA	0.847805	0.015207	0.017844	0.119144	84.28	12.52	3.07	14	LP2 (2026-09-30)	PASS	NonPolyA_perM 14 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5211758	M	M	NA	Agree	0.00	3.81	0.00	19.33	4.60	36.15	NONE	POLYA	0.854864	0.015586	0.018709	0.11084	84.71	12.14	3.01	8	LP2 (2026-09-30)	PASS	NonPolyA_perM 8 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5211759	M	M	NA	Agree	0.00	4.83	0.00	21.75	5.44	33.33	NONE	POLYA	0.930347	0.010367	0.016767	0.042519	91.16	5.35	3.35	11	LP2 (2026-09-30)	PASS	NonPolyA_perM 11 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5211760	M	M	NA	Agree	0.00	3.82	0.00	17.34	4.75	31.52	NONE	POLYA	0.856298	0.011458	0.016627	0.115617	84.61	12.40	2.89	13	LP2 (2026-09-30)	PASS	NonPolyA_perM 13 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5211761	M	M	NA	Agree	0.01	3.02	0.00	20.35	5.27	39.24	NONE	POLYA	0.966064	0.012497	0.019408	0.002031	95.12	1.81	2.95	11	LP2 (2026-09-30)	PASS	NonPolyA_perM 11 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5211762	M	M	NA	Agree	0.00	3.32	0.00	13.03	2.50	35.79	NONE	POLYA	0.867436	0.011451	0.016742	0.104371	85.49	11.38	3.02	20	LP2 (2026-09-30)	PASS	NonPolyA_perM 20 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5211763	M	M	NA	Agree	0.00	4.13	0.00	19.76	5.43	40.16	NONE	POLYA	0.877876	0.017685	0.019716	0.084723	88.17	9.40	2.30	13	LP2 (2026-09-30)	PASS	NonPolyA_perM 13 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5211764	M	M	NA	Agree	0.00	2.48	0.00	11.27	2.57	39.28	NONE	POLYA	0.854526	0.012032	0.017202	0.11624	85.02	12.40	2.48	11	LP2 (2026-09-30)	PASS	NonPolyA_perM 11 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5211765	M	M	NA	Agree	0.00	3.76	0.00	16.07	4.30	44.16	NONE	POLYA	0.840811	0.017149	0.018413	0.123627	84.35	12.95	2.58	17	LP2 (2026-09-30)	PASS	NonPolyA_perM 17 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5211766	M	M	NA	Agree	0.00	3.71	0.00	16.37	3.35	40.57	NONE	POLYA	0.850809	0.015379	0.017134	0.116679	84.44	12.77	2.70	20	LP2 (2026-09-30)	PASS	NonPolyA_perM 20 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does