Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM5105121	F	F	NA	Agree	241.51	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.901042	0.063366	0.030233	0.005359	95.26	2.80	1.59	79	LP2 (2026-09-30)	PASS	NonPolyA_perM 79 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5105122	F	F	NA	Agree	184.44	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.903435	0.059265	0.029223	0.008076	95.25	2.92	1.52	87	LP2 (2026-09-30)	PASS	NonPolyA_perM 87 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5105123	F	F	NA	Agree	132.46	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.920125	0.046544	0.025838	0.007493	95.14	2.98	1.49	75	LP2 (2026-09-30)	PASS	NonPolyA_perM 75 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5105124	F	F	NA	Agree	286.31	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.878491	0.080962	0.034306	0.006241	94.65	3.28	1.66	110	LP2 (2026-09-30)	PASS	NonPolyA_perM 110 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5105125	F	F	NA	Agree	237.28	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.893061	0.069446	0.032674	0.004818	94.71	3.23	1.65	100	LP2 (2026-09-30)	PASS	NonPolyA_perM 100 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5105126	F	F	NA	Agree	223.62	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.89149	0.065506	0.030389	0.012614	94.30	3.84	1.47	88	LP2 (2026-09-30)	PASS	NonPolyA_perM 88 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5105127	F	F	NA	Agree	80.75	0.00	0.00	0.05	0.00	0.09	NONE	POLYA	0.863656	0.105838	0.029305	0.001201	85.38	1.96	12.24	150	LP2 (2026-09-30)	PASS	NonPolyA_perM 150 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5105128	F	F	NA	Agree	166.59	0.00	0.00	0.03	0.00	0.06	NONE	POLYA	0.780045	0.18474	0.031559	0.003657	87.24	2.78	9.26	326	LP2 (2026-09-30)	PASS	NonPolyA_perM 326 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5105129	F	M	NA	Conflict	0.22	3.47	0.00	18.72	12.82	37.40	NONE	POLYA	0.882182	0.08673	0.029545	0.001544	83.82	1.88	13.89	128	LP2 (2026-09-30)	PASS	NonPolyA_perM 128 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5105130	F	M	NA	Conflict	0.13	3.18	0.00	22.01	14.22	42.24	NONE	POLYA	0.882465	0.083329	0.032275	0.001931	86.95	1.91	10.71	105	LP2 (2026-09-30)	PASS	NonPolyA_perM 105 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5105131	F	M	NA	Conflict	0.11	1.73	0.00	18.54	12.19	27.59	NONE	POLYA	0.883205	0.08628	0.029046	0.001468	85.05	1.86	12.65	107	LP2 (2026-09-30)	PASS	NonPolyA_perM 107 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does