Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-20) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM5061947	M	M	NA	Agree	0.01	8.64	0.00	37.11	19.81	69.35	NONE	POLYA	0.867361	0.08249	0.047878	0.002271	91.81	2.33	5.59	91	LP2 (2026-09-30)	PASS	NonPolyA_perM 91 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5061948	M	M	NA	Agree	0.11	8.51	0.00	43.21	18.65	61.41	NONE	POLYA	0.839457	0.109104	0.049472	0.001967	92.78	1.95	4.99	119	LP2 (2026-09-30)	PASS	NonPolyA_perM 119 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5061949	M	M	NA	Agree	0.02	7.91	0.00	42.73	20.08	66.83	NONE	POLYA	0.84041	0.105956	0.049372	0.004262	90.05	2.27	7.38	96	LP2 (2026-09-30)	PASS	NonPolyA_perM 96 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5061950	M	M	NA	Agree	0.04	7.22	0.00	37.37	17.57	71.43	NONE	POLYA	0.867303	0.082374	0.047275	0.003049	91.61	2.29	5.82	98	LP2 (2026-09-30)	PASS	NonPolyA_perM 98 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5061951	M	M	NA	Agree	0.14	6.30	0.00	39.16	20.04	67.57	NONE	POLYA	0.850242	0.097723	0.049703	0.002331	91.83	2.14	5.73	90	LP2 (2026-09-30)	PASS	NonPolyA_perM 90 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5061952	M	M	NA	Agree	0.02	8.03	0.00	49.82	19.21	70.27	NONE	POLYA	0.853559	0.097391	0.046795	0.002255	92.57	2.11	4.97	98	LP2 (2026-09-30)	PASS	NonPolyA_perM 98 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does