Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-22) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM5032234	F	F	NA	Agree	15.43	0.00	0.00	0.02	0.00	0.04	NONE	POLYA	0.848298	0.050372	0.033718	0.067612	88.98	7.70	3.05	59	LP2 (2026-09-30)	PASS	NonPolyA_perM 59 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5032235	F	F	NA	Agree	26.80	0.00	0.00	0.00	0.00	0.03	NONE	POLYA	0.917372	0.04879	0.032202	0.001637	94.71	2.03	3.00	52	LP2 (2026-09-30)	PASS	NonPolyA_perM 52 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5032236	F	F	NA	Agree	17.73	0.00	0.00	0.00	0.00	0.02	NONE	POLYA	0.836534	0.051496	0.032189	0.079781	87.95	8.60	3.19	61	LP2 (2026-09-30)	PASS	NonPolyA_perM 61 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5032237	F	F	NA	Agree	24.94	0.00	0.00	0.02	0.01	0.04	NONE	POLYA	0.909147	0.058024	0.031576	0.001253	94.90	2.02	2.85	54	LP2 (2026-09-30)	PASS	NonPolyA_perM 54 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5032238	F	F	NA	Agree	19.18	0.00	0.00	0.00	0.01	0.10	NONE	POLYA	0.853211	0.054154	0.031373	0.061262	89.95	6.90	2.93	74	LP2 (2026-09-30)	PASS	NonPolyA_perM 74 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5032239	M	M	NA	Agree	0.01	1.14	0.00	7.51	6.10	29.19	NONE	POLYA	0.936962	0.034254	0.027294	0.001491	94.61	1.85	3.29	26	LP2 (2026-09-30)	PASS	NonPolyA_perM 26 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5032240	M	M	NA	Agree	0.02	0.95	0.00	7.09	4.69	19.71	NONE	POLYA	0.813851	0.063513	0.027209	0.095428	87.20	9.70	2.86	55	LP2 (2026-09-30)	PASS	NonPolyA_perM 55 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5032241	M	M	NA	Agree	0.02	1.16	0.00	8.29	6.37	21.78	NONE	POLYA	0.824743	0.064233	0.030159	0.080866	88.07	8.51	3.19	63	LP2 (2026-09-30)	PASS	NonPolyA_perM 63 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5032242	M	M	NA	Agree	0.02	1.79	0.00	12.84	6.74	55.50	NONE	POLYA	0.860821	0.03533	0.029185	0.074664	88.49	8.05	3.27	34	LP2 (2026-09-30)	PASS	NonPolyA_perM 34 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5032243	F	Undetermined	NA	Conflict	0.52	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.96072	0.012836	0.023236	0.003209	93.34	2.37	3.99	16	LP2 (2026-09-30)	PASS	NonPolyA_perM 16 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5032244	F	Undetermined	NA	Conflict	0.48	0.00	0.00	0.00	0.00	0.02	NONE	POLYA	0.959984	0.015255	0.022089	0.002672	93.26	2.25	4.11	17	LP2 (2026-09-30)	PASS	NonPolyA_perM 17 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5032245	F	F	NA	Agree	22.30	0.00	0.00	0.02	0.00	0.06	NONE	POLYA	0.837751	0.050526	0.03034	0.081383	88.15	8.70	2.91	51	LP2 (2026-09-30)	PASS	NonPolyA_perM 51 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5032246	F	F	NA	Agree	21.31	0.00	0.00	0.06	0.04	0.08	NONE	POLYA	0.878176	0.029128	0.036374	0.056322	89.88	6.53	3.41	43	LP2 (2026-09-30)	PASS	NonPolyA_perM 43 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5032247	M	M	NA	Agree	0.00	0.03	0.00	0.51	1.55	2.43	NONE	POLYA	0.843218	0.0319	0.02272	0.102162	86.56	10.37	2.86	39	LP2 (2026-09-30)	PASS	NonPolyA_perM 39 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5032248	M	M	NA	Agree	0.01	0.89	0.00	5.29	3.98	19.09	NONE	POLYA	0.89384	0.074621	0.029691	0.001848	94.50	1.89	3.33	52	LP2 (2026-09-30)	PASS	NonPolyA_perM 52 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5032249	M	M	NA	Agree	0.00	0.38	0.00	1.99	2.22	8.57	NONE	POLYA	0.92228	0.048578	0.026405	0.002737	94.42	2.07	3.23	43	LP2 (2026-09-30)	PASS	NonPolyA_perM 43 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5032250	M	M	NA	Agree	0.01	2.13	0.00	15.48	8.39	68.09	NONE	POLYA	0.869446	0.025464	0.028041	0.077049	88.25	8.26	3.31	28	LP2 (2026-09-30)	PASS	NonPolyA_perM 28 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM5032251	M	M	NA	Agree	0.00	2.86	0.00	17.98	9.74	57.98	NONE	POLYA	0.831904	0.068468	0.033189	0.066438	89.06	7.42	3.33	79	LP2 (2026-09-30)	PASS	NonPolyA_perM 79 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does