Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM4982793	M	M	NA	Agree	0.00	1.87	0.00	13.28	2.10	13.32	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.363892	0.050832	0.044758	0.540518	27.78	55.87	15.35	7713	LP2 (2026-09-30)	LOW_MAPPING	NonPolyA_perM 7713 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM4982794	M	M	NA	Agree	0.04	2.72	0.00	13.38	3.11	14.17	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.345295	0.045053	0.037408	0.572243	24.90	57.76	16.55	6206	LP2 (2026-09-30)	LOW_MAPPING	NonPolyA_perM 6206 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM4982795	M	M	NA	Agree	0.01	2.21	0.00	11.93	2.52	15.37	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.364986	0.027054	0.04381	0.56415	29.58	58.01	11.63	5179	LP2 (2026-09-30)	LOW_MAPPING	NonPolyA_perM 5179 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM4982796	M	M	NA	Agree	0.00	3.17	0.00	18.94	4.51	20.73	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.432644	0.047375	0.04401	0.475971	37.94	48.85	12.56	3956	LP2 (2026-09-30)	LOW_MAPPING	NonPolyA_perM 3956 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM4982797	M	M	NA	Agree	0.03	3.31	0.00	18.76	4.50	21.03	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.450318	0.054291	0.046067	0.449324	33.81	46.54	18.85	4292	LP2 (2026-09-30)	LOW_MAPPING	NonPolyA_perM 4292 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM4982798	M	M	NA	Agree	0.01	2.66	0.00	15.19	4.12	14.68	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.423432	0.060675	0.048239	0.467653	34.79	47.36	17.12	5954	LP2 (2026-09-30)	LOW_MAPPING	NonPolyA_perM 5954 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not