Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-20) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM4947463	M	M	NA	Agree	0.03	7.49	0.00	48.02	24.46	49.88	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.863078	0.095534	0.039982	0.001405	92.91	3.39	3.08	123	LP2 (2026-09-30)	PASS	NonPolyA_perM 123 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4947464	M	M	NA	Agree	0.04	9.40	0.00	48.88	31.44	60.32	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.859234	0.097452	0.0414	0.001913	92.35	3.39	3.68	110	LP2 (2026-09-30)	PASS	NonPolyA_perM 110 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4947465	M	M	NA	Agree	0.06	9.04	0.00	50.62	27.82	59.33	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.867909	0.089315	0.041249	0.001526	93.47	3.40	2.58	90	LP2 (2026-09-30)	PASS	NonPolyA_perM 90 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4947466	M	M	NA	Agree	0.01	7.15	0.00	43.16	20.51	46.86	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.864003	0.090381	0.041874	0.003742	93.25	3.49	2.76	107	LP2 (2026-09-30)	PASS	NonPolyA_perM 107 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4947467	M	M	NA	Agree	0.03	5.67	0.00	37.21	20.38	41.53	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.848179	0.100385	0.04428	0.007156	92.61	3.73	3.15	129	LP2 (2026-09-30)	PASS	NonPolyA_perM 129 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4947468	M	M	NA	Agree	0.01	6.12	0.00	35.48	21.34	40.12	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.85103	0.106489	0.04101	0.001471	94.16	3.09	2.24	122	LP2 (2026-09-30)	PASS	NonPolyA_perM 122 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does