Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-20) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM4890287	F	F	NA	Agree	171.00	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.796018	0.130126	0.055863	0.017993	89.49	4.34	5.75	135	LP2 (2026-09-30)	PASS	NonPolyA_perM 135 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4890288	F	F	NA	Agree	170.12	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.798167	0.130009	0.053903	0.017921	89.08	4.30	6.19	132	LP2 (2026-09-30)	PASS	NonPolyA_perM 132 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4890289	F	F	NA	Agree	153.12	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.80559	0.12232	0.054411	0.01768	89.79	4.27	5.51	125	LP2 (2026-09-30)	PASS	NonPolyA_perM 125 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4890290	F	F	NA	Agree	162.38	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.798025	0.127968	0.054558	0.019449	89.27	4.46	5.85	123	LP2 (2026-09-30)	PASS	NonPolyA_perM 123 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4890291	F	F	NA	Agree	159.75	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.803476	0.122834	0.054729	0.01896	89.28	4.44	5.86	116	LP2 (2026-09-30)	PASS	NonPolyA_perM 116 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4890292	F	F	NA	Agree	164.06	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.804212	0.122872	0.054662	0.018255	88.61	4.34	6.62	127	LP2 (2026-09-30)	PASS	NonPolyA_perM 127 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4890293	F	F	NA	Agree	183.09	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.794326	0.133333	0.0549	0.017441	89.35	4.23	5.98	149	LP2 (2026-09-30)	PASS	NonPolyA_perM 149 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4890294	F	F	NA	Agree	158.82	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.799607	0.126771	0.055013	0.018609	89.40	4.44	5.72	126	LP2 (2026-09-30)	PASS	NonPolyA_perM 126 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4890295	F	F	NA	Agree	180.76	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.805629	0.122327	0.05385	0.018194	89.40	4.34	5.82	131	LP2 (2026-09-30)	PASS	NonPolyA_perM 131 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4890296	F	F	NA	Agree	157.60	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.801734	0.123715	0.05367	0.020881	88.72	4.60	6.25	134	LP2 (2026-09-30)	PASS	NonPolyA_perM 134 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4890297	F	F	NA	Agree	181.96	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.793559	0.130026	0.053786	0.022629	89.32	4.61	5.63	138	LP2 (2026-09-30)	PASS	NonPolyA_perM 138 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4890298	F	F	NA	Agree	171.36	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.795806	0.126269	0.054074	0.023852	88.84	4.84	5.88	127	LP2 (2026-09-30)	PASS	NonPolyA_perM 127 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4890299	F	F	NA	Agree	149.96	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.801213	0.128826	0.05371	0.016251	89.89	4.09	5.59	121	LP2 (2026-09-30)	PASS	NonPolyA_perM 121 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4890300	F	F	NA	Agree	185.30	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.800327	0.125748	0.055052	0.018873	89.62	4.44	5.47	128	LP2 (2026-09-30)	PASS	NonPolyA_perM 128 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4890301	F	F	NA	Agree	223.60	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.785812	0.141353	0.056469	0.016366	89.74	4.15	5.66	141	LP2 (2026-09-30)	PASS	NonPolyA_perM 141 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4890302	F	F	NA	Agree	239.30	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.789886	0.138201	0.054868	0.017045	90.16	4.14	5.26	148	LP2 (2026-09-30)	PASS	NonPolyA_perM 148 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4890303	F	F	NA	Agree	192.50	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.789354	0.138017	0.053157	0.019472	89.33	4.41	5.81	132	LP2 (2026-09-30)	PASS	NonPolyA_perM 132 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4890304	F	F	NA	Agree	199.41	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.791065	0.139559	0.053197	0.016179	90.01	4.08	5.48	140	LP2 (2026-09-30)	PASS	NonPolyA_perM 140 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4890305	F	F	NA	Agree	188.85	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.797333	0.13443	0.053507	0.01473	90.10	3.97	5.48	136	LP2 (2026-09-30)	PASS	NonPolyA_perM 136 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4890306	F	F	NA	Agree	189.82	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.80456	0.128191	0.053457	0.013792	89.60	3.82	6.14	127	LP2 (2026-09-30)	PASS	NonPolyA_perM 127 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4890307	F	F	NA	Agree	206.40	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.791845	0.135366	0.053512	0.019277	89.39	4.32	5.86	145	LP2 (2026-09-30)	PASS	NonPolyA_perM 145 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4890308	F	F	NA	Agree	179.22	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.80274	0.127201	0.054386	0.015674	90.13	4.07	5.34	126	LP2 (2026-09-30)	PASS	NonPolyA_perM 126 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4890309	F	F	NA	Agree	172.99	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.804877	0.12588	0.05345	0.015793	89.68	4.05	5.84	128	LP2 (2026-09-30)	PASS	NonPolyA_perM 128 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4890310	F	F	NA	Agree	166.39	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.80281	0.127818	0.054259	0.015114	89.24	3.98	6.34	140	LP2 (2026-09-30)	PASS	NonPolyA_perM 140 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4890311	F	F	NA	Agree	194.62	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.797719	0.129979	0.055176	0.017127	89.75	4.27	5.54	131	LP2 (2026-09-30)	PASS	NonPolyA_perM 131 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4890312	F	F	NA	Agree	170.43	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.793782	0.13236	0.053554	0.020304	89.24	4.44	5.89	132	LP2 (2026-09-30)	PASS	NonPolyA_perM 132 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4890313	F	F	NA	Agree	204.54	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.789148	0.138372	0.055168	0.017312	89.76	4.22	5.57	134	LP2 (2026-09-30)	PASS	NonPolyA_perM 134 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4890314	F	F	NA	Agree	183.59	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.801017	0.126849	0.053831	0.018303	89.61	4.33	5.62	116	LP2 (2026-09-30)	PASS	NonPolyA_perM 116 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4890315	F	F	NA	Agree	211.48	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.79098	0.136515	0.055438	0.017068	89.36	4.17	6.02	134	LP2 (2026-09-30)	PASS	NonPolyA_perM 134 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4890317	F	F	NA	Agree	190.75	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.796728	0.133494	0.053701	0.016077	89.46	4.09	5.98	126	LP2 (2026-09-30)	PASS	NonPolyA_perM 126 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4890318	F	F	NA	Agree	207.11	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.796625	0.132849	0.054082	0.016444	89.87	4.13	5.54	124	LP2 (2026-09-30)	PASS	NonPolyA_perM 124 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4890319	F	F	NA	Agree	184.20	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.80611	0.12406	0.053576	0.016254	90.05	4.16	5.35	129	LP2 (2026-09-30)	PASS	NonPolyA_perM 129 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4890320	F	F	NA	Agree	130.98	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.803417	0.126092	0.052804	0.017688	90.05	4.21	5.31	124	LP2 (2026-09-30)	PASS	NonPolyA_perM 124 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4890321	F	F	NA	Agree	205.54	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.799371	0.131927	0.053805	0.014898	90.31	3.98	5.25	138	LP2 (2026-09-30)	PASS	NonPolyA_perM 138 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4890322	F	F	NA	Agree	186.86	0.00	0.00	0.00	0.00	0.00	NONE	POLYA	0.793182	0.133666	0.053125	0.020026	89.65	4.45	5.44	128	LP2 (2026-09-30)	PASS	NonPolyA_perM 128 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does