Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM4829857	M	M	NA	Agree	0.01	0.93	0.00	6.85	5.21	34.18	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.920304	0.043308	0.035007	0.001381	79.75	1.78	18.18	84	LP2 (2026-09-30)	PASS	NonPolyA_perM 84 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4829858	M	M	NA	Agree	0.11	6.04	1.13	44.02	33.82	80.64	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.840163	0.113263	0.045676	0.000898	85.72	1.91	11.93	104	LP2 (2026-09-30)	PASS	NonPolyA_perM 104 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4829859	M	M	NA	Agree	0.09	6.79	0.00	38.12	27.63	81.09	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.885191	0.071105	0.036145	0.007559	78.07	2.38	19.21	81	LP2 (2026-09-30)	PASS	NonPolyA_perM 81 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4829860	M	M	NA	Agree	0.19	6.78	0.00	45.27	31.38	68.59	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.884786	0.074505	0.035376	0.005333	81.83	2.25	15.57	78	LP2 (2026-09-30)	PASS	NonPolyA_perM 78 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4829861	M	M	NA	Agree	0.03	4.83	0.00	37.25	25.61	70.85	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.870977	0.086707	0.040949	0.001368	83.61	1.84	14.18	75	LP2 (2026-09-30)	PASS	NonPolyA_perM 75 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4829862	M	M	NA	Agree	0.03	5.50	0.00	33.01	42.63	77.27	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.868762	0.08254	0.039684	0.009015	85.91	2.59	11.05	59	LP2 (2026-09-30)	PASS	NonPolyA_perM 59 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4829863	M	M	NA	Agree	0.02	5.76	2.11	44.74	29.01	80.47	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.874762	0.081205	0.03743	0.006603	83.64	2.30	13.71	89	LP2 (2026-09-30)	PASS	NonPolyA_perM 89 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4829864	M	M	NA	Agree	0.09	6.65	0.00	41.91	33.27	72.44	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.843066	0.113057	0.042717	0.00116	85.96	1.90	11.65	100	LP2 (2026-09-30)	PASS	NonPolyA_perM 100 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4829865	M	M	NA	Agree	0.10	8.18	4.79	44.03	36.72	91.70	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.841246	0.113094	0.044658	0.001001	85.33	2.01	12.06	112	LP2 (2026-09-30)	PASS	NonPolyA_perM 112 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does