Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM4811724	M	M	NA	Agree	0.05	11.71	0.00	47.93	25.67	67.38	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.887272	0.077035	0.034997	0.000696	94.08	2.64	2.88	73	LP2 (2026-09-30)	PASS	NonPolyA_perM 73 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4811725	M	M	NA	Agree	0.06	11.70	0.67	47.60	28.37	67.24	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.886032	0.078942	0.034285	0.000741	93.99	2.63	2.99	77	LP2 (2026-09-30)	PASS	NonPolyA_perM 77 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4811726	M	M	NA	Agree	0.05	9.81	0.00	38.07	20.77	74.73	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.916624	0.050092	0.032275	0.00101	93.87	2.86	2.85	53	LP2 (2026-09-30)	PASS	NonPolyA_perM 53 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4811727	M	M	NA	Agree	0.03	11.17	0.00	51.05	24.53	58.87	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.896845	0.065922	0.036412	0.000821	94.23	2.47	2.89	67	LP2 (2026-09-30)	PASS	NonPolyA_perM 67 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4811728	M	M	NA	Agree	0.07	13.47	0.00	56.89	28.20	55.42	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.878838	0.082967	0.037635	0.000559	94.17	2.49	2.85	73	LP2 (2026-09-30)	PASS	NonPolyA_perM 73 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4811729	M	M	NA	Agree	0.03	11.26	0.00	46.77	19.50	60.33	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.918167	0.045146	0.035059	0.001629	93.99	2.76	2.83	54	LP2 (2026-09-30)	PASS	NonPolyA_perM 54 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4811730	M	M	NA	Agree	0.02	10.79	0.00	46.70	21.85	65.61	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.899196	0.065091	0.034922	0.000791	94.01	2.67	2.83	50	LP2 (2026-09-30)	PASS	NonPolyA_perM 50 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4811731	M	M	NA	Agree	0.04	9.74	0.00	46.09	25.00	68.72	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.90022	0.064539	0.03453	0.000712	94.09	2.65	2.85	57	LP2 (2026-09-30)	PASS	NonPolyA_perM 57 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4811732	M	M	NA	Agree	0.05	9.13	0.00	43.26	19.01	55.28	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.902158	0.066429	0.030587	0.000826	94.22	2.55	2.84	47	LP2 (2026-09-30)	PASS	NonPolyA_perM 47 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does