Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM4762294	not_specified	F	NA	Conflict	68.40	0.85	0.00	5.76	4.11	7.76	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.84198	0.09953	0.051482	0.007008	67.35	2.01	30.40	87	LP2 (2026-09-30)	PASS	NonPolyA_perM 87 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4762295	not_specified	M	NA	Conflict	0.19	3.43	0.00	30.56	15.59	49.86	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.889428	0.058916	0.046534	0.005122	74.82	2.08	22.95	62	LP2 (2026-09-30)	PASS	NonPolyA_perM 62 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4762296	not_specified	F	NA	Conflict	49.42	0.26	0.00	1.71	0.44	2.04	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.894196	0.052916	0.04491	0.007978	69.75	2.17	27.93	65	LP2 (2026-09-30)	PASS	NonPolyA_perM 65 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4762297	not_specified	F	NA	Conflict	46.16	0.00	0.00	0.24	0.50	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.881904	0.05362	0.051067	0.013408	56.15	2.26	41.41	71	LP2 (2026-09-30)	PASS	NonPolyA_perM 71 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4762298	not_specified	F	NA	Conflict	24.89	0.05	0.00	1.20	0.11	0.60	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.887475	0.048576	0.057454	0.006495	63.03	2.16	34.64	59	LP2 (2026-09-30)	PASS	NonPolyA_perM 59 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4762299	not_specified	M	NA	Conflict	0.00	2.46	0.00	19.76	12.13	38.11	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.87476	0.058172	0.061693	0.005375	59.89	1.85	37.97	65	LP2 (2026-09-30)	PASS	NonPolyA_perM 65 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4762300	not_specified	M	NA	Conflict	0.01	3.65	0.00	23.71	13.76	41.05	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.900692	0.04446	0.051082	0.003767	66.46	1.94	31.46	59	LP2 (2026-09-30)	PASS	NonPolyA_perM 59 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4762301	not_specified	M	NA	Conflict	0.02	1.44	0.00	14.18	11.61	28.38	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.899054	0.040644	0.056373	0.003929	61.08	1.97	36.77	66	LP2 (2026-09-30)	PASS	NonPolyA_perM 66 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4762302	not_specified	M	NA	Conflict	4.29	4.12	0.00	26.26	16.12	46.47	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.887398	0.060672	0.049386	0.002544	84.31	2.08	13.47	64	LP2 (2026-09-30)	PASS	NonPolyA_perM 64 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4762303	not_specified	M	NA	Conflict	0.01	4.69	0.00	27.92	19.32	62.19	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.893971	0.053179	0.050356	0.002495	85.82	2.22	11.79	52	LP2 (2026-09-30)	PASS	NonPolyA_perM 52 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4762304	not_specified	F	NA	Conflict	31.64	0.05	0.00	1.08	0.42	2.60	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.894768	0.050354	0.049325	0.005554	76.58	2.30	20.93	66	LP2 (2026-09-30)	PASS	NonPolyA_perM 66 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4762305	not_specified	F	NA	Conflict	50.67	0.02	0.00	1.23	0.72	1.79	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.885047	0.059358	0.052724	0.002871	82.71	2.17	14.96	65	LP2 (2026-09-30)	PASS	NonPolyA_perM 65 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4762306	not_specified	F	NA	Conflict	18.82	0.00	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.894237	0.051369	0.051717	0.002677	87.39	2.54	9.92	65	LP2 (2026-09-30)	PASS	NonPolyA_perM 65 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4762307	not_specified	F	NA	Conflict	61.05	0.00	0.00	0.04	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.883059	0.063968	0.049981	0.002992	85.67	2.14	12.03	83	LP2 (2026-09-30)	PASS	NonPolyA_perM 83 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4762308	not_specified	F	NA	Conflict	48.84	0.09	0.00	0.00	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.87565	0.058716	0.062978	0.002655	73.28	2.01	24.39	60	LP2 (2026-09-30)	PASS	NonPolyA_perM 60 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4762309	not_specified	M	NA	Conflict	1.95	4.23	0.00	27.00	16.53	43.99	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.894941	0.054519	0.046999	0.003541	78.03	2.02	19.80	76	LP2 (2026-09-30)	PASS	NonPolyA_perM 76 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4762310	not_specified	M	NA	Conflict	6.87	3.84	0.00	33.78	15.90	46.05	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.889186	0.059319	0.04687	0.004625	85.72	2.27	11.87	66	LP2 (2026-09-30)	PASS	NonPolyA_perM 66 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4762311	not_specified	M	NA	Conflict	0.01	3.13	0.00	27.42	19.24	50.22	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.896002	0.055799	0.044	0.004199	83.67	2.05	14.13	62	LP2 (2026-09-30)	PASS	NonPolyA_perM 62 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4762312	not_specified	F	NA	Conflict	40.33	0.12	0.00	1.76	1.54	3.25	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.885908	0.060926	0.045174	0.007992	76.25	2.37	21.22	62	LP2 (2026-09-30)	PASS	NonPolyA_perM 62 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4762313	not_specified	F	NA	Conflict	22.40	0.00	0.00	0.96	0.35	2.55	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.903532	0.037987	0.045765	0.012716	68.38	2.66	28.83	52	LP2 (2026-09-30)	PASS	NonPolyA_perM 52 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4762314	not_specified	M	NA	Conflict	0.00	3.20	0.00	27.68	19.28	35.89	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.875027	0.070081	0.050323	0.004569	76.35	2.08	21.40	99	LP2 (2026-09-30)	PASS	NonPolyA_perM 99 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4762315	not_specified	M	NA	Conflict	0.01	2.43	0.00	22.54	14.78	36.11	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.873386	0.073153	0.047167	0.006295	77.24	2.20	20.40	87	LP2 (2026-09-30)	PASS	NonPolyA_perM 87 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4762316	not_specified	F	NA	Conflict	44.99	0.14	0.00	0.32	0.45	1.70	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.879637	0.063766	0.049279	0.007318	71.91	2.25	25.68	75	LP2 (2026-09-30)	PASS	NonPolyA_perM 75 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4762317	not_specified	F	NA	Conflict	54.03	0.00	0.00	0.15	0.00	0.00	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.872704	0.066027	0.055392	0.005877	68.66	2.12	29.08	97	LP2 (2026-09-30)	PASS	NonPolyA_perM 97 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does