Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-20) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM4675510	M	M	NA	Agree	0.00	3.17	0.00	15.25	5.24	39.74	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.942113	0.029516	0.023503	0.004868	94.49	3.82	1.46	19	LP2 (2026-09-30)	PASS	NonPolyA_perM 19 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4675511	M	M	NA	Agree	0.01	5.62	0.00	25.20	6.86	53.36	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.937481	0.03041	0.028398	0.00371	95.15	3.37	1.22	22	LP2 (2026-09-30)	PASS	NonPolyA_perM 22 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4675512	M	M	NA	Agree	0.00	4.96	0.00	21.08	6.39	49.34	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.93376	0.036367	0.02314	0.006733	95.05	3.25	1.45	30	LP2 (2026-09-30)	PASS	NonPolyA_perM 30 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4675513	M	M	NA	Agree	0.01	4.36	0.00	19.54	5.79	47.25	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.940597	0.030395	0.023469	0.00554	94.60	3.56	1.60	21	LP2 (2026-09-30)	PASS	NonPolyA_perM 21 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4675514	M	M	NA	Agree	0.00	3.77	0.00	15.77	5.33	42.64	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.94272	0.027642	0.024012	0.005627	94.62	3.35	1.80	21	LP2 (2026-09-30)	PASS	NonPolyA_perM 21 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4675515	M	M	NA	Agree	0.01	5.13	0.00	20.65	6.98	46.40	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.929869	0.038761	0.026479	0.00489	95.15	3.15	1.47	29	LP2 (2026-09-30)	PASS	NonPolyA_perM 29 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4675516	M	M	NA	Agree	0.01	4.28	0.00	22.03	5.24	51.18	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.935496	0.034979	0.024142	0.005383	95.18	3.27	1.30	28	LP2 (2026-09-30)	PASS	NonPolyA_perM 28 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4675517	M	M	NA	Agree	0.03	4.68	0.00	22.03	6.45	52.08	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.937203	0.033175	0.024852	0.00477	94.92	3.44	1.35	30	LP2 (2026-09-30)	PASS	NonPolyA_perM 30 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4675518	M	M	NA	Agree	0.01	5.57	0.00	26.11	7.06	48.43	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.925314	0.039844	0.028143	0.0067	94.71	3.76	1.26	31	LP2 (2026-09-30)	PASS	NonPolyA_perM 31 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4675519	M	M	NA	Agree	0.00	4.65	0.00	23.75	6.36	54.85	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.926032	0.040874	0.028032	0.005062	94.81	3.54	1.31	32	LP2 (2026-09-30)	PASS	NonPolyA_perM 32 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4675520	M	M	NA	Agree	0.02	3.06	0.00	18.99	5.30	42.69	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.937931	0.032705	0.025004	0.00436	95.01	3.44	1.29	23	LP2 (2026-09-30)	PASS	NonPolyA_perM 23 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4675521	M	M	NA	Agree	0.01	4.30	0.00	18.85	4.70	50.63	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.932117	0.03712	0.026396	0.004366	94.36	3.43	1.95	28	LP2 (2026-09-30)	PASS	NonPolyA_perM 28 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4675522	M	M	NA	Agree	0.01	4.39	0.00	22.03	8.42	47.65	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.925786	0.03922	0.03187	0.003124	93.55	4.45	1.57	41	LP2 (2026-09-30)	PASS	NonPolyA_perM 41 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4675523	M	M	NA	Agree	0.02	4.19	0.00	18.47	5.82	47.26	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.928316	0.040447	0.02592	0.005316	94.79	3.63	1.31	35	LP2 (2026-09-30)	PASS	NonPolyA_perM 35 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4675524	M	M	NA	Agree	0.02	3.95	0.00	20.71	5.13	46.12	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.929586	0.039752	0.025988	0.004673	94.67	3.58	1.49	36	LP2 (2026-09-30)	PASS	NonPolyA_perM 36 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4675525	M	M	NA	Agree	0.02	4.94	0.00	22.85	5.93	54.58	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.936119	0.033493	0.025617	0.00477	94.65	3.50	1.60	29	LP2 (2026-09-30)	PASS	NonPolyA_perM 29 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does