Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-20) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM4648635	M	M	NA	Agree	0.06	4.89	0.00	34.66	12.09	57.94	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.909358	0.042079	0.043283	0.00528	93.85	2.18	3.80	41	LP2 (2026-09-30)	PASS	NonPolyA_perM 41 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4648636	M	M	NA	Agree	0.09	5.63	0.00	34.16	11.95	60.47	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.920833	0.031978	0.042361	0.004828	94.50	2.17	3.16	32	LP2 (2026-09-30)	PASS	NonPolyA_perM 32 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4648637	M	M	NA	Agree	0.04	4.91	0.00	41.52	11.42	60.39	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.909769	0.042901	0.042188	0.005143	94.17	2.11	3.56	39	LP2 (2026-09-30)	PASS	NonPolyA_perM 39 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4648638	M	M	NA	Agree	0.03	5.31	0.00	41.65	10.96	58.51	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.918809	0.033497	0.041782	0.005911	94.44	2.24	3.16	29	LP2 (2026-09-30)	PASS	NonPolyA_perM 29 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4648639	M	M	NA	Agree	0.03	6.04	0.00	41.57	15.03	69.57	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.919988	0.030725	0.043676	0.005611	93.51	2.22	4.08	19	LP2 (2026-09-30)	PASS	NonPolyA_perM 19 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4648640	M	M	NA	Agree	0.07	6.92	0.00	48.44	13.75	77.35	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.919835	0.030773	0.045123	0.00427	93.09	2.11	4.63	26	LP2 (2026-09-30)	PASS	NonPolyA_perM 26 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4648641	M	M	NA	Agree	0.05	5.39	0.00	36.70	12.38	65.93	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.922337	0.029166	0.042886	0.005611	93.77	2.18	3.89	22	LP2 (2026-09-30)	PASS	NonPolyA_perM 22 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4648642	M	M	NA	Agree	0.12	5.03	0.00	36.75	12.16	63.77	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.921061	0.03134	0.042129	0.00547	94.10	2.15	3.58	27	LP2 (2026-09-30)	PASS	NonPolyA_perM 27 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4648643	M	M	NA	Agree	0.04	5.27	0.00	35.57	12.28	60.52	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.909785	0.038961	0.04413	0.007125	94.11	2.34	3.39	39	LP2 (2026-09-30)	PASS	NonPolyA_perM 39 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4648644	M	M	NA	Agree	0.07	4.56	0.00	32.73	13.03	54.43	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.917468	0.034189	0.042929	0.005414	94.54	2.19	3.12	27	LP2 (2026-09-30)	PASS	NonPolyA_perM 27 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4648645	M	M	NA	Agree	0.05	4.74	0.00	37.87	13.75	59.01	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.912789	0.03945	0.042118	0.005644	94.26	2.13	3.45	28	LP2 (2026-09-30)	PASS	NonPolyA_perM 28 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4648646	M	M	NA	Agree	0.03	4.97	0.00	39.13	13.06	62.50	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.919919	0.032289	0.042074	0.005718	94.44	2.17	3.24	26	LP2 (2026-09-30)	PASS	NonPolyA_perM 26 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does