Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-20) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM4633490	M	M	NA	Agree	0.16	9.69	0.00	52.08	21.96	68.06	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.850387	0.09995	0.049219	0.000444	94.52	2.16	3.04	92	LP2 (2026-09-30)	PASS	NonPolyA_perM 92 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4633491	M	M	NA	Agree	0.11	9.78	0.00	49.41	21.45	68.27	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.850812	0.100343	0.048488	0.000356	93.84	2.19	3.69	75	LP2 (2026-09-30)	PASS	NonPolyA_perM 75 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4633492	M	M	NA	Agree	0.25	9.46	0.00	48.22	21.85	65.38	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.848785	0.101429	0.049295	0.000491	93.99	2.24	3.52	95	LP2 (2026-09-30)	PASS	NonPolyA_perM 95 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4633493	M	M	NA	Agree	0.02	9.21	0.00	48.47	27.49	76.72	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.845025	0.105321	0.049265	0.000389	94.54	2.12	3.08	102	LP2 (2026-09-30)	PASS	NonPolyA_perM 102 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4633494	M	M	NA	Agree	0.09	8.09	0.00	52.64	16.46	69.03	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.855695	0.094698	0.048911	0.000696	93.23	2.16	4.34	83	LP2 (2026-09-30)	PASS	NonPolyA_perM 83 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4633495	M	M	NA	Agree	0.16	7.05	0.00	52.29	18.94	66.24	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.861246	0.09014	0.048116	0.000498	94.07	2.16	3.51	91	LP2 (2026-09-30)	PASS	NonPolyA_perM 91 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4633496	M	M	NA	Agree	0.20	8.56	0.00	53.62	20.58	69.20	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.849278	0.100254	0.049824	0.000643	93.34	2.17	4.23	89	LP2 (2026-09-30)	PASS	NonPolyA_perM 89 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4633497	M	M	NA	Agree	0.13	7.95	0.00	48.18	21.13	76.32	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.868876	0.081314	0.048829	0.000981	93.85	2.21	3.72	69	LP2 (2026-09-30)	PASS	NonPolyA_perM 69 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4633498	M	M	NA	Agree	0.13	7.92	0.00	54.62	20.15	69.92	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.867905	0.082592	0.049002	0.0005	93.59	2.16	3.99	76	LP2 (2026-09-30)	PASS	NonPolyA_perM 76 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4633499	M	M	NA	Agree	0.17	8.39	0.00	47.83	22.03	73.79	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.861028	0.08889	0.049418	0.000664	93.63	2.17	3.95	79	LP2 (2026-09-30)	PASS	NonPolyA_perM 79 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does