Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-22) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM4508455	M	M	NA	Agree	1.22	33.10	0.00	113.60	40.78	369.46	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.725028	0.205283	0.063077	0.006612	92.67	3.75	3.20	1001	LP2 (2026-09-30)	PASS	NonPolyA_perM 1001 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM4508456	M	M	NA	Agree	1.48	42.42	0.00	111.45	45.95	402.37	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.70072	0.222694	0.066819	0.009768	92.52	3.99	3.08	954	LP2 (2026-09-30)	PASS	NonPolyA_perM 954 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM4508457	M	M	NA	Agree	2.09	44.11	0.00	114.07	50.16	418.99	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.637181	0.286728	0.06491	0.01118	92.51	3.84	3.23	990	LP2 (2026-09-30)	PASS	NonPolyA_perM 990 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM4508458	M	M	NA	Agree	1.56	43.55	0.00	111.01	48.59	397.29	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.692791	0.230039	0.067	0.010171	92.30	4.04	3.26	979	LP2 (2026-09-30)	PASS	NonPolyA_perM 979 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM4508459	M	M	NA	Agree	1.84	45.35	0.00	110.93	48.94	411.61	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.682141	0.244307	0.065899	0.007653	92.50	3.77	3.32	993	LP2 (2026-09-30)	PASS	NonPolyA_perM 993 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not
GSM4508460	M	M	NA	Agree	2.34	45.59	0.00	120.46	52.04	446.04	SECOND_READ_TRANSCRIPTION_STRAND	RRNA_DEPLETED	0.645339	0.287917	0.062758	0.003986	93.20	3.14	3.26	915	LP2 (2026-09-30)	PASS	NonPolyA_perM 915 >= 700: non-polyadenylated small RNAs retained, as rRNA depletion does and oligo-dT selection does not