Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM4504572	M	M	NA	Agree	0.01	9.65	0.00	34.35	9.14	70.01	NONE	POLYA	0.943146	0.018116	0.02411	0.014628	93.89	2.71	3.30	19	LP2 (2026-09-30)	PASS	NonPolyA_perM 19 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4504573	M	M	NA	Agree	0.00	9.53	0.00	40.82	9.77	76.88	NONE	POLYA	0.928577	0.01724	0.019933	0.03425	92.39	4.44	3.08	13	LP2 (2026-09-30)	PASS	NonPolyA_perM 13 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4504574	M	M	NA	Agree	0.00	6.46	0.00	24.47	7.62	52.41	NONE	POLYA	0.92681	0.01487	0.024077	0.034242	91.78	4.50	3.62	28	LP2 (2026-09-30)	PASS	NonPolyA_perM 28 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4504575	M	M	NA	Agree	0.00	7.72	0.00	29.75	8.11	63.60	NONE	POLYA	0.946323	0.017917	0.02555	0.010209	94.14	2.39	3.36	16	LP2 (2026-09-30)	PASS	NonPolyA_perM 16 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4504576	M	M	NA	Agree	0.00	11.88	0.00	33.45	9.40	64.28	NONE	POLYA	0.944492	0.022552	0.027088	0.005868	94.68	1.92	3.29	27	LP2 (2026-09-30)	PASS	NonPolyA_perM 27 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4504577	M	M	NA	Agree	0.00	8.50	0.00	37.89	8.67	70.29	NONE	POLYA	0.951849	0.017804	0.024359	0.005988	94.79	1.95	3.14	15	LP2 (2026-09-30)	PASS	NonPolyA_perM 15 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4504578	M	M	NA	Agree	0.00	8.55	0.00	33.94	9.88	62.70	NONE	POLYA	0.946056	0.019134	0.028237	0.006573	94.47	2.04	3.38	23	LP2 (2026-09-30)	PASS	NonPolyA_perM 23 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4504579	M	M	NA	Agree	0.00	9.17	0.00	34.12	8.80	62.94	NONE	POLYA	0.941699	0.021631	0.028082	0.008588	94.29	2.27	3.30	20	LP2 (2026-09-30)	PASS	NonPolyA_perM 20 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4504580	M	M	NA	Agree	0.00	9.27	0.00	31.37	8.43	53.21	NONE	POLYA	0.947601	0.021331	0.026749	0.004319	95.10	1.83	2.95	27	LP2 (2026-09-30)	PASS	NonPolyA_perM 27 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4504581	M	M	NA	Agree	0.01	9.28	0.00	36.84	9.59	60.00	NONE	POLYA	0.939503	0.02649	0.028	0.006006	95.03	2.00	2.85	24	LP2 (2026-09-30)	PASS	NonPolyA_perM 24 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4504582	M	M	NA	Agree	0.00	8.44	0.00	29.97	8.41	57.37	NONE	POLYA	0.950666	0.018486	0.027209	0.003638	95.12	1.80	2.95	19	LP2 (2026-09-30)	PASS	NonPolyA_perM 19 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4504583	M	M	NA	Agree	0.00	9.42	0.00	32.65	9.61	58.38	NONE	POLYA	0.947116	0.019738	0.027106	0.00604	94.93	2.01	2.93	14	LP2 (2026-09-30)	PASS	NonPolyA_perM 14 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4504584	M	M	NA	Agree	0.00	10.29	0.00	35.72	10.16	62.49	NONE	POLYA	0.928275	0.023126	0.026535	0.022064	93.39	3.36	3.15	24	LP2 (2026-09-30)	PASS	NonPolyA_perM 24 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4504585	M	M	NA	Agree	0.00	9.38	0.00	35.19	9.97	63.25	NONE	POLYA	0.947425	0.022127	0.026643	0.003805	95.31	1.73	2.85	21	LP2 (2026-09-30)	PASS	NonPolyA_perM 21 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4504586	M	M	NA	Agree	0.00	8.82	0.00	30.65	8.95	59.34	NONE	POLYA	0.919273	0.017541	0.02596	0.037226	91.26	4.73	3.91	25	LP2 (2026-09-30)	PASS	NonPolyA_perM 25 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4504587	M	M	NA	Agree	0.00	9.00	0.00	31.87	9.74	61.49	NONE	POLYA	0.93023	0.021219	0.026134	0.022418	93.43	3.50	2.95	13	LP2 (2026-09-30)	PASS	NonPolyA_perM 13 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4504588	M	M	NA	Agree	0.00	5.42	0.00	30.74	6.57	61.21	NONE	POLYA	0.958523	0.013388	0.02026	0.00783	94.83	2.14	2.94	15	LP2 (2026-09-30)	PASS	NonPolyA_perM 15 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4504589	M	M	NA	Agree	0.00	5.85	0.00	21.91	5.94	52.86	NONE	POLYA	0.959072	0.012899	0.020035	0.007994	94.47	2.25	3.19	8	LP2 (2026-09-30)	PASS	NonPolyA_perM 8 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does