Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM4497322	M	M	NA	Agree	0.07	3.89	0.00	27.22	10.33	52.25	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.886409	0.05956	0.046341	0.00769	95.61	2.25	1.97	71	LP2 (2026-09-30)	PASS	NonPolyA_perM 71 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4497323	M	M	NA	Agree	0.01	2.88	0.00	28.45	9.34	51.72	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.892051	0.059921	0.041196	0.006832	94.65	2.04	3.11	64	LP2 (2026-09-30)	PASS	NonPolyA_perM 64 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4497324	M	M	NA	Agree	0.02	4.50	0.00	29.46	8.75	60.24	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.883433	0.064376	0.047808	0.004383	95.55	1.92	2.37	57	LP2 (2026-09-30)	PASS	NonPolyA_perM 57 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4497325	M	M	NA	Agree	0.01	3.46	0.00	28.22	8.55	56.23	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.893554	0.051978	0.047203	0.007266	95.38	2.23	2.23	65	LP2 (2026-09-30)	PASS	NonPolyA_perM 65 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4497326	M	M	NA	Agree	0.02	4.63	0.00	30.86	10.79	55.98	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.885918	0.060944	0.046458	0.006679	95.37	2.18	2.27	69	LP2 (2026-09-30)	PASS	NonPolyA_perM 69 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4497327	M	M	NA	Agree	0.04	4.32	0.00	23.71	10.53	52.67	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.862594	0.076728	0.048719	0.011959	94.53	2.82	2.46	102	LP2 (2026-09-30)	PASS	NonPolyA_perM 102 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4497328	M	M	NA	Agree	0.02	3.00	0.00	15.56	8.01	53.82	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.890312	0.056302	0.040063	0.013323	94.30	3.01	2.52	87	LP2 (2026-09-30)	PASS	NonPolyA_perM 87 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4497329	M	M	NA	Agree	0.03	5.56	0.00	30.27	10.51	66.19	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.894736	0.058612	0.040848	0.005804	95.54	2.23	2.06	73	LP2 (2026-09-30)	PASS	NonPolyA_perM 73 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4497330	M	M	NA	Agree	0.02	4.18	0.00	28.80	9.68	65.26	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.898612	0.049092	0.044707	0.007589	95.08	2.33	2.44	49	LP2 (2026-09-30)	PASS	NonPolyA_perM 49 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4497331	M	M	NA	Agree	0.03	3.79	0.00	20.85	9.21	54.21	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.885929	0.063903	0.044933	0.005235	92.99	2.18	4.67	93	LP2 (2026-09-30)	PASS	NonPolyA_perM 93 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4497332	M	M	NA	Agree	0.01	4.90	0.00	29.74	10.99	48.51	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.851086	0.09441	0.047009	0.007495	94.78	2.25	2.78	96	LP2 (2026-09-30)	PASS	NonPolyA_perM 96 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4497333	M	M	NA	Agree	0.01	5.09	0.00	33.31	10.70	72.59	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.898948	0.05114	0.044303	0.005609	95.29	2.20	2.34	90	LP2 (2026-09-30)	PASS	NonPolyA_perM 90 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does