Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-16) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO). SampleID InputSex ComputedSex XYRatio Agreement Xist Uty Sry Ddx3y Kdm5d Eif2s3y ComputedStrand ComputedLibraryPrep PctMRNA PctIntronic PctIntergenic PctRibosomal UniqPct MultiPct TooShortPct NonPolyA_perM LibraryPrepRule LibraryPrepQC LibraryPrepReason GSM4484012 M M NA Agree 0.05 2.04 0.00 17.52 4.84 27.66 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.928239 0.041876 0.023828 0.006057 90.30 2.87 6.31 46 LP2 (2026-09-30) PASS NonPolyA_perM 46 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4484013 M M NA Agree 0.01 2.48 0.00 21.58 5.83 28.26 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.926636 0.046353 0.022781 0.004229 82.56 2.53 14.58 39 LP2 (2026-09-30) PASS NonPolyA_perM 39 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4484014 M M NA Agree 0.02 1.90 0.00 18.29 6.37 30.06 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.932343 0.038771 0.023286 0.005599 83.78 2.88 12.85 41 LP2 (2026-09-30) PASS NonPolyA_perM 41 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4484015 M M NA Agree 0.04 2.00 0.00 18.32 7.16 31.54 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.923396 0.047105 0.02418 0.005319 82.32 2.84 14.41 42 LP2 (2026-09-30) PASS NonPolyA_perM 42 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4484016 M M NA Agree 0.04 1.80 0.00 16.61 4.87 31.30 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.924066 0.042698 0.02518 0.008056 83.38 3.22 12.94 47 LP2 (2026-09-30) PASS NonPolyA_perM 47 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4484017 M M NA Agree 0.06 1.30 0.00 16.64 5.19 26.09 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.930804 0.038218 0.024118 0.00686 81.59 2.86 15.05 45 LP2 (2026-09-30) PASS NonPolyA_perM 45 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4484018 M M NA Agree 0.00 2.05 0.00 21.27 6.83 30.66 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.921242 0.048974 0.024282 0.005502 81.46 2.84 15.33 35 LP2 (2026-09-30) PASS NonPolyA_perM 35 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4484019 M M NA Agree 0.06 3.22 0.00 26.48 8.88 38.22 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.905727 0.060674 0.028079 0.00552 82.70 3.03 13.83 54 LP2 (2026-09-30) PASS NonPolyA_perM 54 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4484020 M M NA Agree 0.03 2.71 0.00 21.78 7.10 29.59 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.92273 0.048749 0.023967 0.004555 82.62 2.67 14.30 61 LP2 (2026-09-30) PASS NonPolyA_perM 61 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4484021 M M NA Agree 0.05 1.41 0.00 13.35 4.48 28.96 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.931324 0.03768 0.02477 0.006226 80.10 2.99 16.50 32 LP2 (2026-09-30) PASS NonPolyA_perM 32 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4484022 M M NA Agree 0.02 2.01 0.00 15.31 4.82 30.62 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.931022 0.040033 0.025133 0.003812 79.50 2.80 17.20 57 LP2 (2026-09-30) PASS NonPolyA_perM 57 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4484023 M M NA Agree 0.02 2.94 0.00 17.70 4.92 28.83 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.928363 0.041763 0.025626 0.004248 81.50 2.81 15.20 73 LP2 (2026-09-30) PASS NonPolyA_perM 73 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4484024 M M NA Agree 0.02 2.35 0.00 18.98 5.06 33.43 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.934169 0.038171 0.023805 0.003855 88.24 2.75 8.54 45 LP2 (2026-09-30) PASS NonPolyA_perM 45 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4484025 M M NA Agree 0.01 2.39 0.00 18.41 6.00 30.38 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.933016 0.040896 0.023213 0.002876 82.49 2.63 14.38 38 LP2 (2026-09-30) PASS NonPolyA_perM 38 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4484026 M M NA Agree 0.02 1.88 0.00 18.54 5.37 27.17 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.928897 0.041968 0.023756 0.00538 82.12 2.88 14.53 46 LP2 (2026-09-30) PASS NonPolyA_perM 46 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4484027 M M NA Agree 0.04 1.82 0.00 16.65 4.67 28.02 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.941237 0.032597 0.023154 0.003012 82.10 2.88 14.63 38 LP2 (2026-09-30) PASS NonPolyA_perM 38 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4484028 M M NA Agree 0.03 1.94 0.00 17.35 5.11 31.55 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.9267 0.038822 0.024683 0.009795 80.48 3.23 15.90 38 LP2 (2026-09-30) PASS NonPolyA_perM 38 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4484029 M M NA Agree 0.03 2.00 0.00 17.98 4.90 32.10 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.932332 0.039634 0.024085 0.00395 82.66 2.74 14.14 50 LP2 (2026-09-30) PASS NonPolyA_perM 50 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4484030 M M NA Agree 0.03 1.82 0.00 19.17 6.62 31.34 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.91935 0.050608 0.024823 0.005219 82.93 2.86 13.76 44 LP2 (2026-09-30) PASS NonPolyA_perM 44 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4484031 M M NA Agree 0.06 1.51 0.00 17.40 5.16 31.66 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.93441 0.033799 0.025637 0.006153 80.91 3.29 15.31 43 LP2 (2026-09-30) PASS NonPolyA_perM 43 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4484032 M M NA Agree 0.01 1.91 0.00 18.93 5.41 29.57 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.925679 0.043992 0.024276 0.006053 82.49 2.96 13.97 42 LP2 (2026-09-30) PASS NonPolyA_perM 42 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4484033 M M NA Agree 0.01 2.04 0.00 18.58 7.10 26.37 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.91913 0.048322 0.024029 0.008519 80.87 3.33 15.21 53 LP2 (2026-09-30) PASS NonPolyA_perM 53 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4484034 M M NA Agree 0.03 1.73 0.00 18.41 5.65 24.86 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.926261 0.046375 0.022536 0.004828 83.11 2.73 13.82 48 LP2 (2026-09-30) PASS NonPolyA_perM 48 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4484035 M M NA Agree 0.01 1.57 0.00 17.25 6.53 28.10 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.930743 0.040782 0.023261 0.005215 83.73 3.07 12.83 51 LP2 (2026-09-30) PASS NonPolyA_perM 51 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4484036 M M NA Agree 0.06 2.22 0.00 21.07 6.48 27.96 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.921635 0.049847 0.023163 0.005355 82.82 2.84 13.89 47 LP2 (2026-09-30) PASS NonPolyA_perM 47 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4484037 M M NA Agree 0.01 1.79 0.00 12.82 5.53 19.68 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.922934 0.049161 0.022948 0.004958 82.77 2.76 14.03 44 LP2 (2026-09-30) PASS NonPolyA_perM 44 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4484038 M M NA Agree 0.01 1.46 0.00 16.06 5.57 23.56 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.932098 0.037282 0.022825 0.007794 81.31 3.05 15.26 49 LP2 (2026-09-30) PASS NonPolyA_perM 49 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4484039 M M NA Agree 0.01 1.88 0.00 19.48 5.97 29.45 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.927485 0.042552 0.024721 0.005241 82.11 2.92 14.58 48 LP2 (2026-09-30) PASS NonPolyA_perM 48 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4484040 M M NA Agree 0.03 3.28 0.00 22.18 6.52 29.49 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.92303 0.047967 0.023854 0.005149 88.27 2.93 8.29 66 LP2 (2026-09-30) PASS NonPolyA_perM 66 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4484041 M M NA Agree 0.01 2.30 0.00 19.86 7.08 27.22 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.925927 0.044521 0.023252 0.0063 79.15 2.86 17.55 39 LP2 (2026-09-30) PASS NonPolyA_perM 39 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4484042 M M NA Agree 0.01 1.92 0.00 21.17 7.50 28.61 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.921853 0.046917 0.02564 0.00559 83.50 3.04 13.09 37 LP2 (2026-09-30) PASS NonPolyA_perM 37 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4484043 M M NA Agree 0.03 2.25 0.00 20.05 7.18 26.51 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.918367 0.049847 0.025384 0.006402 82.57 2.83 14.16 56 LP2 (2026-09-30) PASS NonPolyA_perM 56 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4484044 M M NA Agree 0.00 4.30 0.00 18.52 11.11 55.82 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.891051 0.065658 0.039424 0.003868 83.14 2.41 14.17 88 LP2 (2026-09-30) PASS NonPolyA_perM 88 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4484045 M M NA Agree 0.04 5.99 0.00 20.69 14.88 72.74 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.868587 0.07267 0.03851 0.020232 78.09 3.94 17.74 87 LP2 (2026-09-30) PASS NonPolyA_perM 87 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4484046 M M NA Agree 0.07 4.07 0.00 14.54 10.91 54.91 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.888614 0.067596 0.04047 0.00332 82.08 2.59 15.05 85 LP2 (2026-09-30) PASS NonPolyA_perM 85 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4484047 M M NA Agree 0.02 3.06 0.00 12.34 9.60 54.14 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.896121 0.06086 0.039393 0.003627 82.92 2.67 14.13 72 LP2 (2026-09-30) PASS NonPolyA_perM 72 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4484048 M M NA Agree 0.04 4.63 0.00 23.00 12.77 59.19 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.88012 0.075377 0.041511 0.002992 80.95 2.39 16.39 91 LP2 (2026-09-30) PASS NonPolyA_perM 91 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4484049 M M NA Agree 0.02 4.56 0.00 19.79 10.95 51.56 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.890492 0.068083 0.038014 0.003412 81.02 2.46 16.25 67 LP2 (2026-09-30) PASS NonPolyA_perM 67 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4484050 M M NA Agree 0.08 5.12 0.00 19.43 10.66 60.66 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.887003 0.069879 0.039456 0.003662 82.66 2.46 14.64 57 LP2 (2026-09-30) PASS NonPolyA_perM 57 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4484051 M M NA Agree 0.05 4.76 0.00 19.93 11.55 56.86 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.839613 0.087135 0.039385 0.033866 79.33 5.01 15.38 76 LP2 (2026-09-30) PASS NonPolyA_perM 76 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4484052 M M NA Agree 0.07 5.78 0.00 22.49 12.37 61.03 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.880818 0.076882 0.039421 0.00288 83.08 2.36 14.30 78 LP2 (2026-09-30) PASS NonPolyA_perM 78 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4484053 M M NA Agree 0.02 3.99 0.00 16.91 11.60 56.67 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.888248 0.070394 0.038414 0.002944 84.80 2.28 12.64 66 LP2 (2026-09-30) PASS NonPolyA_perM 66 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4484054 M M NA Agree 0.01 4.71 0.00 24.48 14.30 56.90 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.875309 0.075788 0.040524 0.00838 82.36 2.84 14.51 66 LP2 (2026-09-30) PASS NonPolyA_perM 66 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4484055 M M NA Agree 0.02 3.61 0.00 19.08 11.64 51.80 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.882993 0.074479 0.039098 0.003429 80.03 2.42 17.29 86 LP2 (2026-09-30) PASS NonPolyA_perM 86 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4484056 M M NA Agree 0.02 4.79 0.00 22.57 12.50 55.30 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.882227 0.073448 0.040969 0.003356 81.50 2.42 15.79 75 LP2 (2026-09-30) PASS NonPolyA_perM 75 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4484057 M M NA Agree 0.01 5.06 0.00 21.94 13.13 48.40 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.88447 0.072675 0.0399 0.002954 85.20 2.44 12.10 68 LP2 (2026-09-30) PASS NonPolyA_perM 68 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4484058 M M NA Agree 0.01 4.25 0.00 17.80 10.36 57.39 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.878953 0.076751 0.040772 0.003524 81.82 2.39 15.50 80 LP2 (2026-09-30) PASS NonPolyA_perM 80 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4484059 M M NA Agree 0.03 5.22 0.00 24.38 13.43 48.43 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.879133 0.077918 0.039946 0.003003 84.20 2.40 13.11 92 LP2 (2026-09-30) PASS NonPolyA_perM 92 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4484060 M M NA Agree 0.04 4.62 0.00 16.99 11.16 57.83 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.882175 0.073461 0.041018 0.003346 83.09 2.41 14.25 75 LP2 (2026-09-30) PASS NonPolyA_perM 75 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4484061 M M NA Agree 0.07 4.83 0.00 18.98 11.55 56.20 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.880439 0.076442 0.039595 0.003523 82.83 2.34 14.58 87 LP2 (2026-09-30) PASS NonPolyA_perM 87 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4484062 M M NA Agree 0.02 4.81 0.00 20.92 13.10 64.91 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.880904 0.074129 0.04078 0.004187 84.18 2.53 13.03 76 LP2 (2026-09-30) PASS NonPolyA_perM 76 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4484063 M M NA Agree 0.00 3.94 0.00 20.04 12.65 64.46 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.871837 0.078356 0.045047 0.00476 84.45 2.56 12.72 76 LP2 (2026-09-30) PASS NonPolyA_perM 76 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4484064 M M NA Agree 0.01 5.39 0.00 24.29 13.62 58.22 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.855579 0.085798 0.055129 0.003495 81.50 2.46 15.75 76 LP2 (2026-09-30) PASS NonPolyA_perM 76 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4484065 M M NA Agree 0.03 5.62 0.00 24.44 11.35 64.09 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.852617 0.085245 0.058219 0.003919 78.21 2.54 18.93 86 LP2 (2026-09-30) PASS NonPolyA_perM 86 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4484066 M M NA Agree 0.03 3.87 0.00 22.33 11.51 64.56 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.858186 0.081765 0.05617 0.003879 82.28 2.49 14.93 66 LP2 (2026-09-30) PASS NonPolyA_perM 66 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4484067 M M NA Agree 0.05 5.88 0.00 22.49 12.27 64.38 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.852826 0.085822 0.057609 0.003743 79.88 2.43 17.41 80 LP2 (2026-09-30) PASS NonPolyA_perM 80 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4484068 M M NA Agree 0.00 3.62 0.00 20.27 10.79 61.02 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.857503 0.084697 0.053911 0.003889 82.21 2.56 14.92 86 LP2 (2026-09-30) PASS NonPolyA_perM 86 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4484069 M M NA Agree 0.10 4.87 0.00 17.63 9.58 42.64 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.844526 0.090592 0.061317 0.003564 79.31 2.36 18.03 71 LP2 (2026-09-30) PASS NonPolyA_perM 71 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4484070 M M NA Agree 0.04 4.30 0.00 20.72 12.48 66.37 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.847174 0.085916 0.059968 0.006942 77.54 2.70 19.49 84 LP2 (2026-09-30) PASS NonPolyA_perM 84 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4484071 M M NA Agree 0.08 4.64 0.00 21.61 11.49 47.41 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.863098 0.080085 0.052398 0.004419 78.51 2.54 18.66 75 LP2 (2026-09-30) PASS NonPolyA_perM 75 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4484072 M M NA Agree 0.02 5.03 0.00 21.33 11.45 64.26 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.853494 0.086827 0.055639 0.004039 78.70 2.45 18.56 72 LP2 (2026-09-30) PASS NonPolyA_perM 72 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4484073 M M NA Agree 0.00 5.19 0.00 24.52 13.20 65.82 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.865214 0.085125 0.045844 0.003817 81.20 2.46 16.05 83 LP2 (2026-09-30) PASS NonPolyA_perM 83 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4484074 M M NA Agree 0.05 5.13 0.00 22.29 12.61 64.91 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.87168 0.078487 0.045497 0.004336 81.14 2.48 16.09 94 LP2 (2026-09-30) PASS NonPolyA_perM 94 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does GSM4484075 M M NA Agree 0.00 6.69 0.00 27.45 11.67 72.34 SECOND_READ_TRANSCRIPTION_STRAND POLYA 0.873221 0.080994 0.042866 0.002919 84.23 2.45 13.02 85 LP2 (2026-09-30) PASS NonPolyA_perM 85 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does