Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-21) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM4452159	M	M	NA	Agree	0.00	8.43	0.00	91.25	29.18	79.61	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.81208	0.132331	0.052354	0.003235	87.20	1.77	10.80	149	LP2 (2026-09-30)	PASS	NonPolyA_perM 149 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4452160	M	M	NA	Agree	0.00	8.25	0.00	83.85	23.52	62.45	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.819652	0.125855	0.051409	0.003084	91.64	1.78	6.35	132	LP2 (2026-09-30)	PASS	NonPolyA_perM 132 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4452161	M	M	NA	Agree	0.00	15.00	0.00	80.16	33.21	118.58	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.813514	0.130034	0.052124	0.004329	90.92	1.92	6.91	133	LP2 (2026-09-30)	PASS	NonPolyA_perM 133 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4452162	M	M	NA	Agree	0.00	11.22	0.00	95.25	42.81	138.41	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.821093	0.123499	0.05306	0.002348	90.53	1.71	7.51	175	LP2 (2026-09-30)	PASS	NonPolyA_perM 175 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4452163	M	M	NA	Agree	0.00	14.62	0.00	78.00	30.77	73.51	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.824167	0.122919	0.050433	0.002482	91.39	1.64	6.74	168	LP2 (2026-09-30)	PASS	NonPolyA_perM 168 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4452164	M	M	NA	Agree	0.00	18.79	0.00	37.36	29.75	108.31	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.821223	0.125133	0.051326	0.002318	90.63	1.61	7.53	117	LP2 (2026-09-30)	PASS	NonPolyA_perM 117 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4452165	M	M	NA	Agree	0.00	2.58	0.00	91.38	41.24	79.84	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.81639	0.126347	0.053877	0.003386	90.97	1.88	6.92	172	LP2 (2026-09-30)	PASS	NonPolyA_perM 172 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4452166	M	M	NA	Agree	0.00	9.18	0.00	27.50	36.66	55.09	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.813234	0.131916	0.052298	0.002553	88.78	1.71	9.25	119	LP2 (2026-09-30)	PASS	NonPolyA_perM 119 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4452167	M	M	NA	Agree	0.00	6.63	0.00	70.74	52.86	32.71	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.811408	0.131224	0.053334	0.004035	90.45	1.81	7.50	143	LP2 (2026-09-30)	PASS	NonPolyA_perM 143 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4452168	M	M	NA	Agree	0.00	25.97	0.00	50.18	0.00	88.62	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.809677	0.13413	0.051666	0.004528	89.24	1.94	8.56	160	LP2 (2026-09-30)	PASS	NonPolyA_perM 160 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4452169	M	M	NA	Agree	0.00	11.63	0.00	37.07	40.44	46.98	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.82339	0.120706	0.051258	0.004646	90.70	1.93	7.13	161	LP2 (2026-09-30)	PASS	NonPolyA_perM 161 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4452170	M	M	NA	Agree	0.00	15.38	0.00	71.53	24.65	41.27	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.820546	0.125967	0.050138	0.00335	89.46	1.83	8.47	142	LP2 (2026-09-30)	PASS	NonPolyA_perM 142 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4452171	M	M	NA	Agree	0.00	5.95	0.00	57.49	25.69	132.57	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.830345	0.117346	0.049877	0.002432	91.43	1.72	6.62	177	LP2 (2026-09-30)	PASS	NonPolyA_perM 177 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does