Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-22) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM4450730	M	M	NA	Agree	0.00	2.25	0.00	12.56	3.09	67.96	NONE	POLYA	0.9521	0.021868	0.025324	0.000708	63.99	1.80	33.80	28	LP2 (2026-09-30)	PASS	NonPolyA_perM 28 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4450731	M	M	NA	Agree	0.00	3.92	0.00	20.49	6.14	45.48	NONE	POLYA	0.91217	0.019162	0.033738	0.034931	89.32	4.91	5.60	33	LP2 (2026-09-30)	PASS	NonPolyA_perM 33 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4450732	M	M	NA	Agree	0.00	4.83	0.00	29.98	8.74	41.72	NONE	POLYA	0.918772	0.033839	0.03013	0.017259	93.92	3.21	2.71	47	LP2 (2026-09-30)	PASS	NonPolyA_perM 47 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4450733	M	M	NA	Agree	0.00	4.10	0.00	21.34	6.51	52.98	NONE	POLYA	0.95487	0.017398	0.027292	0.000441	78.89	1.69	19.10	14	LP2 (2026-09-30)	PASS	NonPolyA_perM 14 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4450734	M	M	NA	Agree	0.00	9.35	0.00	22.84	6.51	34.86	NONE	POLYA	0.897565	0.017343	0.029682	0.05541	64.86	5.00	29.96	28	LP2 (2026-09-30)	PASS	NonPolyA_perM 28 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4450735	M	M	NA	Agree	0.00	5.19	0.00	18.87	7.86	40.00	NONE	POLYA	0.903105	0.019615	0.029567	0.047714	82.67	5.45	11.72	16	LP2 (2026-09-30)	PASS	NonPolyA_perM 16 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does