Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-22) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM4422574	M	M	NA	Agree	0.10	6.24	0.00	23.82	13.64	60.85	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.869244	0.085891	0.039165	0.0057	83.50	2.58	13.67	63	LP2 (2026-09-30)	PASS	NonPolyA_perM 63 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4422575	M	M	NA	Agree	0.05	7.28	0.00	27.97	14.39	58.23	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.864553	0.092047	0.04033	0.00307	83.47	2.43	13.83	79	LP2 (2026-09-30)	PASS	NonPolyA_perM 79 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4422576	M	M	NA	Agree	0.01	5.60	0.00	24.72	12.55	63.27	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.850763	0.103939	0.039669	0.005629	83.99	2.67	13.07	71	LP2 (2026-09-30)	PASS	NonPolyA_perM 71 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4422577	M	M	NA	Agree	0.05	5.17	0.00	18.27	11.84	59.74	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.878833	0.075941	0.03997	0.005256	83.65	2.44	13.66	73	LP2 (2026-09-30)	PASS	NonPolyA_perM 73 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4422578	M	M	NA	Agree	0.05	7.48	0.00	29.98	13.11	60.81	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.853225	0.10096	0.042756	0.003058	85.81	2.24	11.67	80	LP2 (2026-09-30)	PASS	NonPolyA_perM 80 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4422579	M	M	NA	Agree	0.04	4.75	0.00	18.08	10.96	58.59	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.880692	0.076867	0.039609	0.002832	84.48	2.27	12.99	63	LP2 (2026-09-30)	PASS	NonPolyA_perM 63 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4422580	M	M	NA	Agree	0.01	5.34	0.00	26.15	11.81	59.85	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.868731	0.089258	0.039639	0.002371	86.97	2.14	10.63	70	LP2 (2026-09-30)	PASS	NonPolyA_perM 70 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4422581	M	M	NA	Agree	0.03	5.51	0.00	23.35	13.54	58.06	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.871355	0.08562	0.038969	0.004056	88.72	2.35	8.65	81	LP2 (2026-09-30)	PASS	NonPolyA_perM 81 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4422582	M	M	NA	Agree	0.02	4.69	0.00	19.11	11.90	52.44	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.843077	0.087489	0.038115	0.031319	83.95	4.79	10.98	89	LP2 (2026-09-30)	PASS	NonPolyA_perM 89 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4422583	M	M	NA	Agree	0.05	4.73	0.00	20.23	11.21	61.83	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.88237	0.075498	0.038921	0.003211	89.67	2.29	7.79	75	LP2 (2026-09-30)	PASS	NonPolyA_perM 75 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4422584	M	M	NA	Agree	0.03	5.22	0.00	19.58	12.55	52.12	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.864969	0.087533	0.038942	0.008557	88.17	2.98	8.57	69	LP2 (2026-09-30)	PASS	NonPolyA_perM 69 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4422585	M	M	NA	Agree	0.06	6.06	0.00	24.40	12.29	53.38	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.870838	0.086975	0.039291	0.002897	88.46	2.26	9.00	67	LP2 (2026-09-30)	PASS	NonPolyA_perM 67 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4422586	M	M	NA	Agree	0.07	4.79	0.00	20.64	12.47	56.95	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.877158	0.080811	0.039083	0.002948	88.69	2.34	8.71	65	LP2 (2026-09-30)	PASS	NonPolyA_perM 65 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4422587	M	M	NA	Agree	0.04	3.41	0.00	13.62	10.45	48.19	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.883433	0.076491	0.03676	0.003315	88.83	2.27	8.64	59	LP2 (2026-09-30)	PASS	NonPolyA_perM 59 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4422588	M	M	NA	Agree	0.03	4.75	0.00	18.11	9.96	59.74	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.860783	0.083725	0.039908	0.015584	87.83	3.49	8.39	79	LP2 (2026-09-30)	PASS	NonPolyA_perM 79 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4422589	M	M	NA	Agree	0.05	6.07	0.00	23.76	11.65	52.54	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.862998	0.090675	0.041058	0.005269	88.29	2.58	8.88	80	LP2 (2026-09-30)	PASS	NonPolyA_perM 80 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4422590	M	M	NA	Agree	0.07	6.40	0.00	23.29	13.69	57.44	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.872429	0.085064	0.039543	0.002964	88.72	2.43	8.55	76	LP2 (2026-09-30)	PASS	NonPolyA_perM 76 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4422591	M	M	NA	Agree	0.01	5.52	0.00	20.80	11.44	53.49	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.883055	0.076215	0.037836	0.002894	87.55	2.52	9.65	65	LP2 (2026-09-30)	PASS	NonPolyA_perM 65 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4422592	M	M	NA	Agree	0.08	4.51	0.00	22.15	12.44	52.14	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.872052	0.084085	0.038469	0.005394	87.02	2.55	10.15	86	LP2 (2026-09-30)	PASS	NonPolyA_perM 86 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4422593	M	M	NA	Agree	0.07	4.96	0.00	17.67	12.87	54.93	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.872161	0.08397	0.04006	0.003809	86.39	2.31	10.99	72	LP2 (2026-09-30)	PASS	NonPolyA_perM 72 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4422594	M	M	NA	Agree	0.08	4.19	0.00	17.36	9.85	56.17	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.878889	0.076388	0.040218	0.004505	83.46	2.39	13.87	72	LP2 (2026-09-30)	PASS	NonPolyA_perM 72 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4422595	M	M	NA	Agree	0.04	3.59	0.00	17.55	10.84	43.14	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.878015	0.080531	0.038623	0.002831	85.57	2.31	11.82	80	LP2 (2026-09-30)	PASS	NonPolyA_perM 80 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4422596	M	M	NA	Agree	0.03	4.29	0.00	18.56	13.45	61.59	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.874787	0.084626	0.036925	0.003662	86.59	2.35	10.75	100	LP2 (2026-09-30)	PASS	NonPolyA_perM 100 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4422597	M	M	NA	Agree	0.01	1.40	0.00	3.75	5.71	13.22	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.884117	0.041191	0.029349	0.045343	82.07	6.41	11.30	55	LP2 (2026-09-30)	PASS	NonPolyA_perM 55 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4422598	M	M	NA	Agree	0.06	3.22	0.00	16.56	12.45	48.50	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.867412	0.088477	0.039842	0.00427	85.43	2.51	11.78	73	LP2 (2026-09-30)	PASS	NonPolyA_perM 73 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4422599	M	M	NA	Agree	0.04	4.41	0.00	17.93	9.68	59.81	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.862491	0.088976	0.041102	0.007432	85.95	2.81	10.94	75	LP2 (2026-09-30)	PASS	NonPolyA_perM 75 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4422600	M	M	NA	Agree	0.07	4.90	0.00	18.94	11.26	54.29	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.874683	0.081165	0.037901	0.006251	88.75	3.41	7.45	110	LP2 (2026-09-30)	PASS	NonPolyA_perM 110 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4422601	M	M	NA	Agree	0.06	6.01	0.00	22.25	12.92	60.32	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.871215	0.083769	0.04129	0.003725	86.28	2.57	10.87	79	LP2 (2026-09-30)	PASS	NonPolyA_perM 79 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4422602	M	M	NA	Agree	0.01	4.47	0.00	18.54	10.78	57.16	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.876835	0.076516	0.041304	0.005344	86.02	2.64	11.02	56	LP2 (2026-09-30)	PASS	NonPolyA_perM 56 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4422603	M	M	NA	Agree	0.03	4.94	0.00	18.65	11.61	54.34	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.865025	0.087804	0.041918	0.005253	86.16	2.63	10.91	94	LP2 (2026-09-30)	PASS	NonPolyA_perM 94 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does