Workflow: expression values from HPC RGD workflow (processed before workflow 2.1); computed sex, strand and library prep added by RGD attribute backfill 1.0 (2026-10-20) using the workflow 2.1 rules. Note: ComputedSex is called from sex-gene expression: R = (Xist TPM + 1) / (median TPM of Uty, Ddx3y, Kdm5d and Eif2s3y + 1); R >= 10 is called F, R <= 1 is called M, and other values, or samples where Xist and the Y-gene median are both below 1 TPM, are Undetermined. Females express Xist and males the Y-linked genes. Sry is reported but not used in the calculation. XYRatio (X:Y read coverage) is shown for reference only. Agreement compares ComputedSex with InputSex (submitted to GEO).
SampleID	InputSex	ComputedSex	XYRatio	Agreement	Xist	Uty	Sry	Ddx3y	Kdm5d	Eif2s3y	ComputedStrand	ComputedLibraryPrep	PctMRNA	PctIntronic	PctIntergenic	PctRibosomal	UniqPct	MultiPct	TooShortPct	NonPolyA_perM	LibraryPrepRule	LibraryPrepQC	LibraryPrepReason
GSM4174985	M	M	NA	Agree	0.00	5.60	0.00	37.59	9.44	128.22	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.911505	0.031752	0.041321	0.015423	90.18	3.83	5.83	64	LP2 (2026-09-30)	PASS	NonPolyA_perM 64 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4174986	M	M	NA	Agree	0.00	6.92	0.00	41.91	12.82	115.31	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.904634	0.030893	0.045194	0.019279	82.70	3.68	13.45	49	LP2 (2026-09-30)	PASS	NonPolyA_perM 49 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4174987	M	M	NA	Agree	0.00	6.08	0.00	35.55	11.31	116.37	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.908916	0.030925	0.044627	0.015531	92.83	4.05	2.94	71	LP2 (2026-09-30)	PASS	NonPolyA_perM 71 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4174988	M	M	NA	Agree	0.00	6.04	0.00	37.60	11.94	109.58	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.898127	0.032457	0.044183	0.025234	91.18	4.80	3.82	72	LP2 (2026-09-30)	PASS	NonPolyA_perM 72 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4174989	M	M	NA	Agree	0.00	5.94	0.00	37.52	11.48	113.77	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.912525	0.027656	0.04185	0.01797	92.28	4.07	3.47	45	LP2 (2026-09-30)	PASS	NonPolyA_perM 45 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4174990	M	M	NA	Agree	0.01	5.55	0.00	33.31	10.81	103.21	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.907161	0.030565	0.043047	0.019227	91.53	4.26	4.03	74	LP2 (2026-09-30)	PASS	NonPolyA_perM 74 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4174991	M	M	NA	Agree	0.00	6.09	0.00	33.48	9.28	123.54	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.911252	0.031147	0.041993	0.015608	91.52	4.07	4.23	87	LP2 (2026-09-30)	PASS	NonPolyA_perM 87 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4174992	M	M	NA	Agree	0.00	6.26	0.00	34.01	7.32	133.32	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.906927	0.03122	0.039876	0.021977	87.04	4.49	8.30	132	LP2 (2026-09-30)	PASS	NonPolyA_perM 132 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4174993	M	M	NA	Agree	0.00	6.15	0.00	32.97	9.71	109.70	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.90903	0.030901	0.042919	0.017151	92.93	4.15	2.74	75	LP2 (2026-09-30)	PASS	NonPolyA_perM 75 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4174994	M	M	NA	Agree	0.00	5.00	0.00	37.95	10.62	111.76	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.908877	0.027807	0.042492	0.020824	92.05	4.25	3.54	54	LP2 (2026-09-30)	PASS	NonPolyA_perM 54 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4174995	M	M	NA	Agree	0.01	5.85	0.00	41.71	11.70	102.44	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.906238	0.032714	0.044118	0.01693	91.11	3.91	4.82	88	LP2 (2026-09-30)	PASS	NonPolyA_perM 88 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4174996	M	M	NA	Agree	0.00	5.49	0.00	29.76	7.80	118.41	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.912997	0.029427	0.039515	0.018061	90.71	4.33	4.79	89	LP2 (2026-09-30)	PASS	NonPolyA_perM 89 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4174997	M	M	NA	Agree	0.00	6.47	0.00	38.82	10.93	121.44	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.913086	0.02886	0.041703	0.016351	92.28	3.95	3.59	60	LP2 (2026-09-30)	PASS	NonPolyA_perM 60 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4174998	M	M	NA	Agree	0.00	7.57	0.00	41.47	11.48	120.97	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.906943	0.031958	0.042889	0.01821	89.68	4.06	6.08	73	LP2 (2026-09-30)	PASS	NonPolyA_perM 73 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4174999	M	M	NA	Agree	0.00	6.33	0.00	32.53	9.78	112.18	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.898318	0.029789	0.042884	0.029008	89.33	5.16	5.33	79	LP2 (2026-09-30)	PASS	NonPolyA_perM 79 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4175000	M	M	NA	Agree	0.00	5.67	0.00	37.31	12.61	108.70	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.903528	0.032626	0.045872	0.017974	90.66	3.95	5.21	59	LP2 (2026-09-30)	PASS	NonPolyA_perM 59 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4175001	M	M	NA	Agree	0.00	5.00	0.00	33.67	8.07	123.23	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.908878	0.032384	0.040069	0.018669	91.87	4.36	3.58	132	LP2 (2026-09-30)	PASS	NonPolyA_perM 132 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does
GSM4175002	M	M	NA	Agree	0.00	6.14	0.00	34.16	12.02	114.16	SECOND_READ_TRANSCRIPTION_STRAND	POLYA	0.906274	0.031031	0.044799	0.017895	93.30	4.34	2.17	86	LP2 (2026-09-30)	PASS	NonPolyA_perM 86 <= 350: non-polyadenylated small RNAs removed, as oligo-dT (polyA) selection does